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PMID:11069915

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Citation

Deinert, K, Fasiolo, F, Hurt, EC and Simos, G (2001) Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs. J. Biol. Chem. 276:6000-8

Abstract

Eukaryotic aminoacyl-tRNA synthetases, in contrast to their prokaryotic counterparts, are often part of high molecular weight complexes. In yeast, two enzymes, the methionyl- and glutamyl-tRNA synthetases associate in vivo with the tRNA-binding protein Arc1p. To study the assembly and function of this complex, we have reconstituted it in vitro from individually purified recombinant proteins. Our results show that Arc1p can readily bind to either or both of the two enzymes, mediating the formation of the respective binary or ternary complexes. Under competition conditions, Arc1p alone exhibits broad specificity and interacts with a defined set of tRNA species. Nevertheless, the in vitro reconstituted Arc1p-containing enzyme complexes can bind only to their cognate tRNAs and tighter than the corresponding monomeric enzymes. These results demonstrate that the organization of aminoacyl-tRNA synthetases with general tRNA-binding proteins into multimeric complexes can stimulate their catalytic efficiency and, therefore, offer a significant advantage to the eukaryotic cell.

Links

PubMed Online version:10.1074/jbc.M008682200

Keywords

Catalysis; Eukaryotic Cells/enzymology; Evolution, Molecular; Glutamate-tRNA Ligase/metabolism; Macromolecular Substances; Methionine-tRNA Ligase/metabolism; Models, Molecular; Peptide Chain Elongation, Translational; Protein Binding; Protein Conformation; RNA, Transfer/metabolism; RNA, Transfer, Glu/metabolism; RNA, Transfer, Met/metabolism; RNA-Binding Proteins/metabolism; Recombinant Proteins/metabolism; Saccharomyces cerevisiae Proteins; Substrate Specificity; Yeasts

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:SYMC

part_of

GO:0017102: methionyl glutamyl tRNA synthetase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SYMC

involved_in

GO:0006431: methionyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:SYMC

involved_in

GO:0006424: glutamyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:SYEC

involved_in

GO:0006424: glutamyl-tRNA aminoacylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:SYEC

enables

GO:0004818: glutamate-tRNA ligase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:SYEC

part_of

GO:0017102: methionyl glutamyl tRNA synthetase complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:SYEC

involved_in

GO:0006431: methionyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:SYEC

involved_in

GO:0006424: glutamyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:ARC1

enables

GO:0008047: enzyme activator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:ARC1

involved_in

GO:0051351: positive regulation of ligase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ARC1

involved_in

GO:0006431: methionyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete

YEAST:ARC1

involved_in

GO:0006424: glutamyl-tRNA aminoacylation

ECO:0005547: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion

P

Seeded From UniProt

complete


See also

References

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