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PMID:10988072

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Citation

Domeier, ME, Morse, DP, Knight, SW, Portereiko, M, Bass, BL and Mango, SE (2000) A link between RNA interference and nonsense-mediated decay in Caenorhabditis elegans. Science 289:1928-31

Abstract

Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA but then recovered; wild-type animals remained silenced. The levels of target messenger RNAs were restored during recovery, and RNA editing and degradation of the dsRNA were identical to those of the wild type. We suggest that persistence of RNA interference relies on a subset of smg genes.

Links

PubMed

Keywords

Adenosine Deaminase/metabolism; Alleles; Animals; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Caenorhabditis elegans Proteins; Gene Silencing; Helminth Proteins/genetics; Helminth Proteins/metabolism; Mutation; Myosin Heavy Chains/genetics; Myosin Heavy Chains/metabolism; Nonmuscle Myosin Type IIB; Phosphoproteins/genetics; Phosphoproteins/metabolism; RNA Stability; RNA, Double-Stranded/metabolism; RNA, Double-Stranded/pharmacology; RNA, Helminth/metabolism; Reverse Transcriptase Polymerase Chain Reaction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5ECF1

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:RENT1

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q1T6W7

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q9BL68

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:Q9BL69

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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