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PMID:10976049

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Citation

Grandel, H, Draper, BW and Schulte-Merker, S (2000) dackel acts in the ectoderm of the zebrafish pectoral fin bud to maintain AER signaling. Development 127:4169-78

Abstract

Classical embryological studies have implied the existence of an apical ectodermal maintenance factor (AEMF) that sustains signaling from the apical ectodermal ridge (AER) during vertebrate limb development. Recent evidence suggests that AEMF activity is composed of different signals involving both a sonic hedgehog (Shh) signal and a fibroblast growth factor 10 (Fgf10) signal from the mesenchyme. In this study we show that the product of the dackel (dak) gene is one of the components that acts in the epidermis of the zebrafish pectoral fin bud to maintain signaling from the apical fold, which is homologous to the AER of tetrapods. dak acts synergistically with Shh to induce fgf4 and fgf8 expression but independently of Shh in promoting apical fold morphogenesis. The failure of dak mutant fin buds to progress from the initial fin induction phase to the autonomous outgrowth phase causes loss of both AER and Shh activity, and subsequently results in a proximodistal truncation of the fin, similar to the result obtained by ridge ablation experiments in the chicken. Further analysis of the dak mutant phenotype indicates that the activity of the transcription factor engrailed 1 (En1) in the ventral non-ridge ectoderm also depends on a maintenance signal probably provided by the ridge. This result uncovers a new interaction between the AER and the dorsoventral organizer in the zebrafish pectoral fin bud.

Links

PubMed

Keywords

Animals; Ectoderm; Embryonic Induction; Epidermis/embryology; Extremities; Fibroblast Growth Factor 4; Fibroblast Growth Factor 8; Fibroblast Growth Factors/metabolism; Genes, Homeobox; Hedgehog Proteins; Homeodomain Proteins/metabolism; Limb Buds/embryology; Morphogenesis; Mutation; Proteins/metabolism; Proto-Oncogene Proteins/metabolism; Tissue Transplantation; Trans-Activators; Transcription Factors; Zebrafish/embryology; Zebrafish Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:F1QUC2

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-001006-1,ZFIN:ZDB-GENO-980202-872

P

Seeded From UniProt

complete

DANRE:F1QUC2

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-001006-1,ZFIN:ZDB-GENO-980202-1195

P

Seeded From UniProt

complete

DANRE:F1QUC2

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-872

P

Seeded From UniProt

complete

DANRE:F1QUC2

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1195

P

Seeded From UniProt

complete

DANRE:Q5U7A7

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-001006-1,ZFIN:ZDB-GENO-980202-872

P

Seeded From UniProt

complete

DANRE:Q5U7A7

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENE-001006-1,ZFIN:ZDB-GENO-980202-1195

P

Seeded From UniProt

complete

DANRE:Q5U7A7

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-872

P

Seeded From UniProt

complete

DANRE:Q5U7A7

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980202-1195

P

Seeded From UniProt

complete

DANRE:SHH

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-980410-268

P

Seeded From UniProt

complete

DANRE:SHH

involved_in

GO:0033339: pectoral fin development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-070406-1

P

Seeded From UniProt

complete


See also

References

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