GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:10970885

From GONUTS
Jump to: navigation, search
Citation

Bhatt, RS, Tomoda, T, Fang, Y and Hatten, ME (2000) Discoidin domain receptor 1 functions in axon extension of cerebellar granule neurons. Genes Dev. 14:2216-28

Abstract

In the developing cerebellum, granule neuron axon outgrowth is a key step toward establishing proper connections with Purkinje neurons, the principal output neuron of the cerebellum. During a search for genes that function in this process, we identified a receptor tyrosine kinase discoidin domain receptor 1 (DDR1) expressed in granule cells throughout their development. Overexpression of a dominant-negative form of DDR1 in immature granule cells results in severe reduction of neurite outgrowth in vitro, in dissociated primary culture, and in vivo, in organotypic slices of neonatal cerebellum. Granule cells that fail to extend axons are positive for differentiation markers such as TAG-1 and the neuron-specific class III beta-tubulin, suggesting that development is affected after granule cells commit to terminal differentiation. DDR1 activation appears to be mediated by its ligand, collagen, which is localized to the pial layer of the developing cerebellum, thereby leading to granule cell parallel fiber extension. Our results therefore indicate that collagen-DDR1 signaling is essential for granule neuron axon formation and further suggest a unique role of pia in cerebellar cortex histogenesis.

Links

PubMed PMC316891

Keywords

Animals; Axons/metabolism; Blotting, Northern; Cell Adhesion Molecules, Neuronal; Cell Differentiation; Cell Division; Cells, Cultured; Cerebellum/embryology; Cerebellum/metabolism; Collagen/metabolism; Contactin 2; Down-Regulation; Gene Expression Regulation, Developmental; Genes, Dominant; Immunoblotting; In Situ Hybridization; Ligands; Membrane Glycoproteins/metabolism; Mice; Neurons/metabolism; Phosphorylation; Pia Mater/metabolism; Plasmids/metabolism; Receptor Protein-Tyrosine Kinases; Receptors, Mitogen/biosynthesis; Receptors, Mitogen/physiology; Signal Transduction; Tissue Distribution; Tubulin/metabolism; Tyrosine/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:B1AVK5

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:88455
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:F7CK55

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:CO4A1

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88456

P

Seeded From UniProt

complete

MOUSE:CO4A2

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:P70165

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:DDR1

acts_upstream_of_or_within

GO:0061564: axon development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DDR1

acts_upstream_of_or_within

GO:0046777: protein autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DDR1

acts_upstream_of_or_within

GO:0038083: peptidyl-tyrosine autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DDR1

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:DDR1

acts_upstream_of_or_within

GO:1990138: neuron projection extension

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:Q3TNM7

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:88455
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q3TP61

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q61436

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q63ZW6

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q6PFB1

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:88455
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q80V57

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q8BNS7

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q9ESQ1

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:88455
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:Q9ESQ2

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:104688
MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:CO4A3

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:2152695
MGI:MGI:88455
MGI:MGI:88456
MGI:MGI:88467

P

Seeded From UniProt

complete

MOUSE:SHH

acts_upstream_of_or_within

GO:0021924: cell proliferation in external granule layer

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CO1A1

acts_upstream_of_or_within

GO:0038063: collagen-activated tyrosine kinase receptor signaling pathway

ECO:0000266: sequence orthology evidence used in manual assertion

UniProtKB:P02454

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.