PMID:10958687

Citation	Shimizu, K, Chiba, S, Hosoya, N, Kumano, K, Saito, T, Kurokawa, M, Kanda, Y, Hamada, Y and Hirai, H (2000) Binding of Delta1, Jagged1, and Jagged2 to Notch2 rapidly induces cleavage, nuclear translocation, and hyperphosphorylation of Notch2. Mol. Cell. Biol. 20:6913-22
Abstract	Delta1, Jagged1, and Jagged2, commonly designated Delta/Serrate/LAG-2 (DSL) proteins, are known to be ligands for Notch1. However, it has been less understood whether they are ligands for Notch receptors other than Notch1. Meanwhile, ligand-induced cleavage and nuclear translocation of the Notch protein are considered to be fundamental for Notch signaling, yet direct observation of the behavior of the Notch molecule after ligand binding, including cleavage and nuclear translocation, has been lacking. In this report, we investigated these issues for Notch2. All of the three DSL proteins bound to endogenous Notch2 on the surface of BaF3 cells, although characteristics of Jagged2 for binding to Notch2 apparently differed from that of Delta1 and Jagged1. After binding, the three DSL proteins induced cleavage of the membrane-spanning subunit of Notch2 (Notch2(TM)), which occurred within 15 min. In a simultaneous time course, the cleaved fragment of Notch2(TM) was translocated into the nucleus. Interestingly, the cleaved Notch2 fragment was hyperphosphorylated also in a time-dependent manner. Finally, binding of DSL proteins to Notch2 also activated the transcription of reporter genes driven by the RBP-Jkappa-responsive promoter. Together, these data indicate that all of these DSL proteins function as ligands for Notch2. Moreover, the findings of rapid cleavage, nuclear translocation, and phosphorylation of Notch2 after ligand binding facilitate the understanding of the Notch signaling.
Links	PubMed PMC88767
Keywords	Animals; Binding Sites; Biological Transport; CHO Cells; Calcium-Binding Proteins; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Line; Cell Membrane/metabolism; Cell Nucleus/metabolism; Cricetinae; Humans; Intercellular Signaling Peptides and Proteins; Intracellular Fluid/metabolism; Intracellular Signaling Peptides and Proteins; Ligands; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Phosphorylation; Protein Binding; Proteins/genetics; Proteins/metabolism; Receptor, Notch2; Receptors, Cell Surface/genetics; Receptors, Cell Surface/metabolism; Solubility; Transcriptional Activation
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