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PMID:10946292

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Citation

Matsushita, M, Thiel, S, Jensenius, JC, Terai, I and Fujita, T (2000) Proteolytic activities of two types of mannose-binding lectin-associated serine protease. J. Immunol. 165:2637-42

Abstract

Mannose (or mannan)-binding lectin (MBL) is an oligomeric serum lectin that plays a role in innate immunity by activating the complement system. In human, two types of MBL-associated serine protease (MASP-1 and MASP-2) and a truncated protein of MASP-2 (small MBL-associated protein; sMAP or MAp19) are complexed with MBL. To clarify the proteolytic activities of MASP-1 and MASP-2 against C4, C2, and C3, we isolated these two types of MASP in activated forms from human serum by sequential affinity chromatography. On an anti-MASP-1 column, MASP-2 passed through the column in the presence of EDTA and high salt concentration, whereas MASP-1 was retained. Isolated MASP-1 and MASP-2 exhibited proteolytic activities against C3 and C4, respectively. C2 was activated by both MASPs. C1 inhibitor (C1 INH), an inhibitor for C1r and C1s, formed equimolar complexes with MASP-1 and MASP-2 and inhibited their proteolytic activities.

Links

PubMed

Keywords

Binding, Competitive/immunology; Carrier Proteins/metabolism; Collectins; Complement Activation/immunology; Complement C1 Inactivator Proteins/metabolism; Dose-Response Relationship, Immunologic; Enzyme Activation/immunology; Humans; Hydrolysis; Lectins/metabolism; Mannose/metabolism; Mannose-Binding Protein-Associated Serine Proteases; Serine Endopeptidases/isolation & purification; Serine Endopeptidases/metabolism; Serine Proteinase Inhibitors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:MASP2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05155

F

Seeded From UniProt

complete

HUMAN:MASP2

enables

GO:0008233: peptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:IC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O00187

F

Seeded From UniProt

complete

HUMAN:IC1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P48740

F

Seeded From UniProt

complete

HUMAN:MASP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05155

F

Seeded From UniProt

complete

HUMAN:IC1

involved_in

GO:0001869: negative regulation of complement activation, lectin pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:MASP1

enables

GO:0008233: peptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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