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PMID:10934045

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Citation

Ohsawa, K, Imai, Y, Kanazawa, H, Sasaki, Y and Kohsaka, S (2000) Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia. J. Cell. Sci. 113 ( Pt 17):3073-84

Abstract

Ionized calcium binding adaptor molecule 1, Iba1, is an EF hand calcium binding protein whose expression is restricted to macrophages/microglia. In this study, Iba1 was shown to colocalize with F-actin in membrane ruffles induced by macrophage colony-stimulating factor and in phagocytic cups formed during zymosan phagocytosis. Expression of mutant Iba1 carrying either N- or C-terminal deletions or carrying a substitution in the calcium binding domain, suppressed the membrane ruffling and the phagocytosis. These results indicate that Iba1 is a key molecule in membrane ruffling and the phagocytosis of macrophages/microglia. Furthermore, Iba1 colocalized with a small GTPase Rac in the membrane ruffles and the phagocytic cups. The Iba1 mutants also suppressed membrane ruffling induced by dominant active Rac1V12, but do not affect microspikes by Cdc42V12 and stress fibers by RhoAV14. These observations suggest that Iba1 is involved in Rac and calcium signaling pathways.

Links

PubMed

Keywords

Actins/metabolism; Amino Acid Sequence; Animals; Calcium/metabolism; Calcium-Binding Proteins/genetics; Calcium-Binding Proteins/physiology; Cell Line; Cell Membrane/metabolism; Cell Membrane/physiology; Colony-Stimulating Factors/pharmacology; DNA-Binding Proteins; EF Hand Motifs; Humans; Macrophages/physiology; Mice; Microfilament Proteins; Microglia/physiology; Molecular Sequence Data; Mutation; Phagocytosis; Rats; Sequence Alignment; Sequence Deletion; Zymosan/metabolism; cdc42 GTP-Binding Protein/genetics; cdc42 GTP-Binding Protein/physiology; rac1 GTP-Binding Protein/genetics; rac1 GTP-Binding Protein/metabolism; rac1 GTP-Binding Protein/physiology; rhoA GTP-Binding Protein/genetics; rhoA GTP-Binding Protein/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:AIF1

involved_in

GO:0016601: Rac protein signal transduction

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AIF1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AIF1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AIF1

involved_in

GO:0006911: phagocytosis, engulfment

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AIF1

located_in

GO:0030027: lamellipodium

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AIF1

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AIF1

located_in

GO:0001891: phagocytic cup

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AIF1

involved_in

GO:0097178: ruffle assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RAC1

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RAC1

located_in

GO:0001891: phagocytic cup

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RAC1

part_of

GO:0001891: phagocytic cup

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RAC1

involved_in

GO:0006911: phagocytosis, engulfment

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RAC1

part_of

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RAC1

involved_in

GO:0097178: ruffle assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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