GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:10922461
| Citation |
Dorazi, R and Dewar, SJ (2000) Membrane topology of the N-terminus of the Escherichia coli FtsK division protein. FEBS Lett. 478:13-8 |
|---|---|
| Abstract |
The Escherichia coli FtsK protein targets the septum, is essential for cell division and may play a role in DNA partitioning. Computer modelling suggests that the first 180 amino acids of the protein are embedded in the cytoplasmic membrane by up to six transmembrane domains. We demonstrate, using gene fusions, that the N-terminus contains four transmembrane helices that link two periplasmic domains. The first periplasmic domain contains an HEXXH amino acid sequence characteristic of zinc metalloproteases. We show by mutation analysis that the conserved glutamic acid of the HEXXH sequence is essential for FtsK function during septation. |
| Links | |
| Keywords |
Alkaline Phosphatase/genetics; Alkaline Phosphatase/metabolism; Amino Acid Motifs; Amino Acid Substitution; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biological Transport; Cell Division; Cell Membrane/chemistry; Cell Membrane/metabolism; Computer Simulation; Conserved Sequence/genetics; Escherichia coli/chemistry; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutation; Protein Structure, Tertiary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Temperature; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0005887: integral component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005886: plasma membrane |
ECO:0000269: experimental evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005887: integral component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005886: plasma membrane |
ECO:0000269: experimental evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
