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This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.
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PMID:10861017
| Citation |
Miao, EA and Miller, SI (2000) A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc. Natl. Acad. Sci. U.S.A. 97:7539-44 |
|---|---|
| Abstract |
Type III secretion systems (TTSS) are important virulence factors that Gram-negative bacteria use to translocate proteins into the cytoplasm of eukaryotic host cells. Salmonellae encode two virulence-associated TTSS. The Salmonella pathogenicity island 1 (SPI1)-encoded TTSS is active on contact with host cells, whereas the Salmonella pathogenicity island 2 (SPI2)-encoded TTSS is expressed after phagocytosis of bacteria by host cells. Previously, no consensus signal sequence for translocation has been identified among TTSS effector proteins. In this work, seven proteins, termed Salmonella-translocated effectors (STE), are described that contain conserved amino acid sequences that direct translocation by TTSS in Salmonella typhimurium. STE that are coordinately regulated with SPI2 gene expression contain translocation signals that are recognized by the SPI2 but not by the SPI1 TTSS. STE that are constitutively expressed contain signals that direct translocation through both SPI1 and SPI2 TTSS. Of the seven STE examined, SspH1 and SspH2 have been previously shown to be translocated and involved in virulence; SlrP and SifA were identified as virulence factors, but were not previously known to be associated with TTSS; and SseI, SseJ, and SifB were previously unidentified. Three STE genes (sspH1, sspH2, and sseI) are located within temperate bacteriophages, suggesting a common mechanism for the dissemination of more recently evolved STE. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Line; Genes, Bacterial; Humans; Molecular Sequence Data; Open Reading Frames/genetics; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Salmonella typhimurium/pathogenicity; Sequence Alignment; Sequence Analysis, Protein; Virulence/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0030254 |
protein secretion by the type III secretion system |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0020002 |
host cell plasma membrane |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0016567 |
protein ubiquitination |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0009405 |
pathogenesis |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0004842 |
ubiquitin-protein ligase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005623 |
cell |
IDA: Inferred from Direct Assay |
C |
Seeded From UniProt |
complete | |||
| GO:0004842 |
ubiquitin-protein transferase activity |
IDA: Inferred from Direct Assay |
F |
Seeded From UniProt |
complete | |||
See also
References
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