GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:10855792
| Citation |
Zhang, H, Wang, Q, Kajino, K and Greene, MI (2000) VCP, a weak ATPase involved in multiple cellular events, interacts physically with BRCA1 in the nucleus of living cells. DNA Cell Biol. 19:253-63 |
|---|---|
| Abstract |
BRCA1, a breast/ovarian cancer susceptibility gene, undergoes mutations in as many as 50% of familial breast tumors. Recent studies indicate that BRCA1 may be involved in DNA damage repair. Here, we demonstrate that the BRCA1 protein physically associates with valosin-containing protein (VCP), a member of the ATPases associated with a variety of cellular activities (AAA) superfamily. In vitro studies revealed that VCP, via its N- terminal region, binds to amino acid residues 303-625 in the BRCA1 protein. Although found predominantly in the cytoplasm and, less abundantly, in the nucleus, VCP can be translocated from the nucleus after stimulation with epidermal growth factor. Collectively, our results suggest that VCP, by binding to BRCA1, participates in the DNA damage-repair function as an ATP transporter, possibly facilitating the transcription-coupled repair. |
| Links |
PubMed Online version:10.1089/10445490050021168 |
| Keywords |
Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Antibodies, Monoclonal/immunology; BRCA1 Protein/immunology; BRCA1 Protein/metabolism; Binding Sites; Biological Transport; Blotting, Western; Cell Cycle Proteins/metabolism; Cell Nucleus/metabolism; Epidermal Growth Factor/metabolism; Humans; Molecular Weight; Phosphorylation; Precipitin Tests; Sequence Analysis; Tumor Cells, Cultured |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
