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PMID:10851135

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Citation

Kuznicki, KA, Smith, PA, Leung-Chiu, WM, Estevez, AO, Scott, HC and Bennett, KL (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127:2907-16

Abstract

We report that four putative germline RNA helicases, GLHs, are components of the germline-specific P granules in Caenorhabditis elegans. GLH-3 and GLH-4, newly discovered, belong to a multi-gene glh family. Although GLHs are homologous to Drosophila VASA, a polar granule component necessary for oogenesis and embryonic pattern formation, the GLHs are distinguished by containing multiple CCHC zinc fingers. RNA-mediated interference (RNAi) reveals the GLHs are critical for oogenesis. By RNAi at 20 degrees C, when either loss of GLH-1 or GLH-4 alone has no effect, loss of both GLH-1 and GLH-4 results in 97% sterility in the glh-1/4(RNAi) offspring of injected hermaphrodites. glh-1/4(RNAi) germlines are under-proliferated and are without oocytes. glh-1/4(RNAi) animals produce sperm; however, spermatogenesis is delayed and the sperm are defective. P granules are still present in glh-1/4(RNAi) sterile worms as revealed with antibodies against the remaining GLH-2 and GLH-3 proteins, indicating the GLHs function independently in P granule assembly. These studies reveal that C.elegans can use GLH-1 or GLH-4 to promote germline development.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Blotting, Northern; Blotting, Western; Caenorhabditis elegans/embryology; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/physiology; Cell Division/genetics; Cell Division/physiology; Cell Nucleus/metabolism; Immunohistochemistry; In Situ Hybridization; Male; Molecular Sequence Data; Multigene Family; Oogenesis/genetics; Oogenesis/physiology; Phenotype; RNA/metabolism; RNA Helicases/genetics; RNA Helicases/physiology; Sequence Homology, Amino Acid; Spermatogenesis/genetics; Spermatogenesis/physiology; Temperature; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:GLH3

located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GLH4

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GLH4

involved_in

GO:0007276: gamete generation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

CAEEL:GLH4

enables

GO:0003724: RNA helicase activity

ECO:0000250: sequence similarity evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:GLH4

located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:GLH4

involved_in

GO:0042127: regulation of cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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