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PMID:10783239

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Citation

Yakushi, T, Masuda, K, Narita, S, Matsuyama, S and Tokuda, H (2000) A new ABC transporter mediating the detachment of lipid-modified proteins from membranes. Nat. Cell Biol. 2:212-8

Abstract

Lipoproteins in Escherichia coli are anchored to the periplasmic side of either the inner or the outer membrane by a lipid moiety that is covalently attached to the amino-terminal cysteine residue. Membrane specificity depends on a sorting signal at position 2 of the lipoprotein. Lipoproteins directed to the outer membrane are released from the inner membrane in an ATP-dependent manner through the formation of a complex with LolA, a periplasmic chaperone. However, the ATPase involved in this reaction has not been identified. Here we show, using reconstituted proteoliposomes, that a new complex, LolCDE, belonging to the ATP-binding cassette (ABC) transporter family, catalyses the release of lipoproteins in LolA- and sorting-signal-dependent manners. The LolCDE complex differs mechanistically from all other ABC transporters as it is not involved in the transmembrane transport of substrates. This new mechanism is evolutionarily conserved in other gram-negative bacteria.

Links

PubMed Online version:10.1038/35008635

Keywords

ATP-Binding Cassette Transporters/genetics; ATP-Binding Cassette Transporters/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Gene Expression/physiology; Lipid Bilayers/metabolism; Lipoproteins/metabolism; Liposomes/chemistry; Liposomes/metabolism; Molecular Sequence Data; Mutagenesis/physiology; Plasmids; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:LOLC

enables

GO:0140306: lipoprotein releasing activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:LOLC

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLD

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLD

enables

GO:0140306: lipoprotein releasing activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:LOLD

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLE

located_in

GO:0005887: integral component of plasma membrane

ECO:0000255: match to sequence model evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLE

enables

GO:0140306: lipoprotein releasing activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:LOLE

acts_upstream_of_or_within

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLD

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLD

involved_in

GO:0089705: protein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLD

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLD

enables

GO:0005524: ATP binding

ECO:0000255: match to sequence model evidence used in manual assertion

F

Seeded From UniProt

Missing: with/from

ECOLI:LOLD

part_of

GO:0098797: plasma membrane protein complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLD

GO:0043190: ATP-binding cassette (ABC) transporter complex

ECO:0000314:

C

Fig1 C

complete
CACAO 10232

ECOLI:LOLD

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig1 C

complete
CACAO 10233

ECOLI:LOLD

GO:0044874: lipoprotein localization to outer membrane

ECO:0000315:

P

Fig3, C

complete
CACAO 10367

ECOLI:LOLD

GO:0005886: plasma membrane

ECO:0000314:

C

Fig2C

complete
CACAO 10454

ECOLI:LOLD

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLC

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLC

part_of

GO:0098797: plasma membrane protein complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLC

GO:0005886: plasma membrane

ECO:0000314:

C

Fig2C

complete
CACAO 10458

ECOLI:LOLE

part_of

GO:0098797: plasma membrane protein complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ECOLI:LOLE

involved_in

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LOLE

part_of

GO:0005887: integral component of plasma membrane

ECO:0000255: match to sequence model evidence used in manual assertion

C

Seeded From UniProt

Missing: with/from

ECOLI:LOLE

GO:0089705: protein localization to outer membrane

ECO:0000314:

P

Fig1C

complete
CACAO 10455

ECOLI:LOLE

GO:0044874: lipoprotein localization to outer membrane

ECO:0000314:

P

Fig1C

complete
CACAO 10456

ECOLI:LOLE

GO:0005886: plasma membrane

ECO:0000314:

C

Fig2C

complete
CACAO 10457

Notes

See also

References

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