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PMID:10781593

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Citation

Hong, Y, Maeda, Y, Watanabe, R, Inoue, N, Ohishi, K and Kinoshita, T (2000) Requirement of PIG-F and PIG-O for transferring phosphoethanolamine to the third mannose in glycosylphosphatidylinositol. J. Biol. Chem. 275:20911-9

Abstract

Many eukaryotic proteins are anchored by glycosylphosphatidylinositol (GPI) to the cell surface membrane. The GPI anchor is linked to proteins by an amide bond formed between the carboxyl terminus and phosphoethanolamine attached to the third mannose. Here, we report the roles of two mammalian genes involved in transfer of phosphoethanolamine to the third mannose in GPI. We cloned a mouse gene termed Pig-o that encodes a 1101-amino acid PIG-O protein bearing regions conserved in various phosphodiesterases. Pig-o knockout F9 embryonal carcinoma cells expressed very little GPI-anchored proteins and accumulated the same major GPI intermediate as the mouse class F mutant cell, which is defective in transferring phosphoethanolamine to the third mannose due to mutant Pig-f gene. PIG-O and PIG-F proteins associate with each other, and the stability of PIG-O was dependent upon PIG-F. However, the class F cell is completely deficient in the surface expression of GPI-anchored proteins. A minor GPI intermediate seen in Pig-o knockout but not class F cells had more than three mannoses with phosphoethanolamines on the first and third mannoses, suggesting that this GPI may account for the low expression of GPI-anchored proteins. Therefore, mammalian cells have redundant activities in transferring phosphoethanolamine to the third mannose, both of which require PIG-F.

Links

PubMed Online version:10.1074/jbc.M001913200

Keywords

Amino Acid Sequence; Animals; CHO Cells; Cloning, Molecular; Cricetinae; Dolichol Monophosphate Mannose/metabolism; Flow Cytometry; Glycosylphosphatidylinositols/biosynthesis; Glycosylphosphatidylinositols/genetics; Glycosylphosphatidylinositols/metabolism; Mannose/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Mice; Molecular Sequence Data; Mutation; Phosphatidylethanolamines/metabolism; Proteins/metabolism; Recombination, Genetic; Sequence Alignment; Transfection; Tumor Cells, Cultured

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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