GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:10769179

From GONUTS
Jump to: navigation, search
Citation

Curie, C, Alonso, JM, Le Jean, M, Ecker, JR and Briat, JF (2000) Involvement of NRAMP1 from Arabidopsis thaliana in iron transport. Biochem. J. 347 Pt 3:749-55

Abstract

Nramp genes code for a widely distributed class of proteins involved in a variety of processes, ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast. Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions, including iron. In plants, iron transport was shown to require a reduction/Fe(II) transport system. In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes. Here we report the sequence of five NRAMP proteins from A. thaliana. Sequence comparison suggests that there are two classes of NRAMP proteins in plants: A. thaliana (At) NRAMP1 and Oriza sativa (Os) NRAMP1 and 3 (two rice isologues) represent one class, and AtNRAMP2-5 and OsNRAMP2 the other. AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low- and high-affinity iron transports, whereas AtNramp2 and OsNramp2 fail to do so. In addition, AtNramp1 transcript, but not AtNramp2 transcript, accumulates in response to iron deficiency in roots but not in leaves. Finally, overexpression of AtNramp1 in transgenic A. thaliana plants leads to an increase in plant resistance to toxic iron concentration. Taken together, these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants.

Links

PubMed PMC1221012

Keywords

Amino Acid Sequence; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis/metabolism; Biological Transport/drug effects; Carrier Proteins/chemistry; Carrier Proteins/classification; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cation Transport Proteins; Cloning, Molecular; Conserved Sequence/genetics; Gene Expression Regulation, Plant/drug effects; Genes, Fungal/genetics; Genes, Plant/genetics; Genes, Plant/physiology; Genetic Complementation Test; Homeostasis; Iron/metabolism; Iron/pharmacology; Iron-Binding Proteins; Membrane Proteins/chemistry; Membrane Proteins/classification; Membrane Proteins/genetics; Membrane Proteins/metabolism; Membrane Transport Proteins; Molecular Sequence Data; Multigene Family/genetics; Mutation/genetics; Oryza sativa/genetics; Phylogeny; Plant Proteins/chemistry; Plant Proteins/classification; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Genetically Modified; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Plant/genetics; RNA, Plant/metabolism; Saccharomyces cerevisiae/drug effects; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ORYSJ:NRAM1

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ORYSJ:NRAM1

involved_in

GO:0034755: iron ion transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NRAM1

enables

GO:0005381: iron ion transmembrane transporter activity

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004662
SGD:S000004938

F

Seeded From UniProt

complete

ARATH:NRAM1

acts_upstream_of_or_within

GO:0034755: iron ion transmembrane transport

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004662
SGD:S000004938

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.