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PMID:10766846

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Citation

Konishi, M, Mikami, T, Yamasaki, M, Miyake, A and Itoh, N (2000) Fibroblast growth factor-16 is a growth factor for embryonic brown adipocytes. J. Biol. Chem. 275:12119-22

Abstract

In rat embryos, fibroblast growth factor (FGF)-16 is predominantly expressed in brown adipose tissue. To elucidate the role of FGF-16, we examined the expression of FGF-16 mRNA in rat embryonic brown adipose tissue at different developmental stages by Northern blotting analysis and in situ hybridization. FGF-16 mRNA was expressed abundantly in brown adipose tissue during embryonic day 17. 5, embryonic days 17.5-19.5, and thereafter at lower levels into the neonatal period. The expression profile of FGF-16 mRNA well corresponds to the proliferative profile of embryonic brown adipose tissue reported. We also examined the mitogenic activity of recombinant rat FGF-16 for primary brown adipocytes prepared from rat embryonic brown adipose tissue. FGF-16 showed significant mitogenic activity for primary brown adipocytes. The mitogenic activity was found to be exerted by binding and activating FGF receptor-4 in the brown adipose tissue. As a great induction of proliferation of rat brown adipose tissue during cold acclimation was reported, we also examined the expression of FGF-16 mRNA in the brown adipose tissue during cold acclimation by Northern blotting analysis. The expression of FGF-16 mRNA was not increased, but rather decreased. The expression profile of FGF-16 mRNA and the mitogenic activity of FGF-16 reported here indicate that FGF-16 is a unique growth factor involved in proliferation of embryonic brown adipose tissue.

Links

PubMed

Keywords

Adipose Tissue, Brown/embryology; Animals; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Division; Cold Temperature; Fibroblast Growth Factors/physiology; In Situ Hybridization; Ion Channels; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mitochondrial Proteins; RNA, Messenger/metabolism; Rats; Rats, Wistar; Uncoupling Agents/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:FGF2

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q498D6

P

Seeded From UniProt

complete

RAT:FGF2

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P21803

P

Seeded From UniProt

complete

RAT:FGF2

involved_in

GO:0008543: fibroblast growth factor receptor signaling pathway

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35758

P

Seeded From UniProt

complete

RAT:FGF2

enables

GO:0005104: fibroblast growth factor receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q498D6

F

Seeded From UniProt

complete

RAT:FGF2

enables

GO:0005104: fibroblast growth factor receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P21803

F

Seeded From UniProt

complete

RAT:FGF2

enables

GO:0005104: fibroblast growth factor receptor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O35758

F

Seeded From UniProt

complete


See also

References

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