PMID:10749931

Citation	Ouspenski, II, Cabello, OA and Brinkley, BR (2000) Chromosome condensation factor Brn1p is required for chromatid separation in mitosis. Mol. Biol. Cell 11:1305-13
Abstract	This work describes BRN1, the budding yeast homologue of Drosophila Barren and Xenopus condensin subunit XCAP-H. The Drosophila protein is required for proper chromosome segregation in mitosis, and Xenopus protein functions in mitotic chromosome condensation. Mutant brn1 cells show a defect in mitotic chromosome condensation and sister chromatid separation and segregation in anaphase. Chromatid cohesion before anaphase is properly maintained in the mutants. Some brn1 mutant cells apparently arrest in S-phase, pointing to a possible function for Brn1p at this stage of the cell cycle. Brn1p is a nuclear protein with a nonuniform distribution pattern, and its level is up-regulated at mitosis. Temperature-sensitive mutations of BRN1 can be suppressed by overexpression of a novel gene YCG1, which is homologous to another Xenopus condensin subunit, XCAP-G. Overexpression of SMC2, a gene necessary for chromosome condensation, and a homologue of the XCAP-E condensin, does not suppress brn1, pointing to functional specialization of components of the condensin complex.
Links	PubMed PMC14848
Keywords	Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Chromatids/physiology; Chromosomes/physiology; DNA, Fungal/analysis; Drosophila Proteins; In Situ Hybridization, Fluorescence; Mitosis/genetics; Mitosis/physiology; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism
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