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PMID:10747030

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Citation

Longman, D, Johnstone, IL and Cáceres, JF (2000) Functional characterization of SR and SR-related genes in Caenorhabditis elegans. EMBO J. 19:1625-37

Abstract

The SR proteins constitute a family of nuclear phosphoproteins, which are required for constitutive splicing and also influence alternative splicing regulation. Initially, it was suggested that SR proteins were functionally redundant in constitutive splicing. However, differences have been observed in alternative splicing regulation, suggesting unique functions for individual SR proteins. Homology searches of the Caenorhabditis elegans genome identified seven genes encoding putative orthologues of the human factors SF2/ASF, SRp20, SC35, SRp40, SRp75 and p54, and also several SR-related genes. To address the issue of functional redundancy, we used dsRNA interference (RNAi) to inhibit specific SR protein function during C.elegans development. RNAi with CeSF2/ASF caused late embryonic lethality, suggesting that this gene has an essential function during C.elegans development. RNAi with other SR genes resulted in no obvious phenotype, which is indicative of gene redundancy. Simultaneous interference of two or more SR proteins in certain combinations caused lethality or other developmental defects. RNAi with CeSRPK, an SR protein kinase, resulted in early embryonic lethality, suggesting an essential role for SR protein phosphorylation during development.

Links

PubMed PMC310231 Online version:10.1093/emboj/19.7.1625

Keywords

Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/embryology; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; DNA Primers/genetics; Genes, Helminth; Helminth Proteins/genetics; Helminth Proteins/metabolism; Heterogeneous-Nuclear Ribonucleoproteins; Humans; Molecular Sequence Data; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phosphorylation; RNA Splicing; RNA, Helminth/genetics; RNA, Helminth/metabolism; RNA-Binding Proteins; Ribonucleoproteins/genetics; Ribonucleoproteins/metabolism; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


See also

References

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