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PMID:10692449
| Citation |
Brenner, R, Jegla, TJ, Wickenden, A, Liu, Y and Aldrich, RW (2000) Cloning and functional characterization of novel large conductance calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4. J. Biol. Chem. 275:6453-61 |
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| Abstract |
We present the cloning and characterization of two novel calcium-activated potassium channel beta subunits, hKCNMB3 and hKCNMB4, that are enriched in the testis and brain, respectively. We compare and contrast the steady state and kinetic properties of these beta subunits with the previously cloned mouse beta1 (mKCNMB1) and the human beta2 subunit (hKCNMB2). Once inactivation is removed, we find that hKCNMB2 has properties similar to mKCNMB1. hKCNMB2 slows Hslo1 channel gating and shifts the current-voltage relationship to more negative potentials. hKCNMB3 and hKCNMB4 have distinct effects on slo currents not observed with mKCNMB1 and hKCNMB2. Although we found that hKCNMB3 does interact with Hslo channels, its effects on Hslo1 channel properties were slight, increasing Hslo1 activation rates. In contrast, hKCNMB4 slows Hslo1 gating kinetics, and modulates the apparent calcium sensitivity of Hslo1. We found that the different effects of the beta subunits on some Hslo1 channel properties are calcium-dependent. mKCNMB1 and hKCNMB2 slow activation at 1 microM but not at 10 microM free calcium concentrations. hKCNMB4 decreases Hslo1 channel openings at low calcium concentrations but increases channel openings at high calcium concentrations. These results suggest that beta subunits in diverse tissue types fine-tune slo channel properties to the needs of a particular cell. |
| Links | |
| Keywords |
Amino Acid Sequence; Brain/metabolism; Calcium/pharmacology; Cloning, Molecular; Humans; Ion Channel Gating; Kinetics; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; Large-Conductance Calcium-Activated Potassium Channel beta Subunits; Large-Conductance Calcium-Activated Potassium Channels; Male; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Patch-Clamp Techniques; Potassium Channels/chemistry; Potassium Channels/genetics; Potassium Channels, Calcium-Activated; RNA, Messenger/metabolism; Sequence Alignment; Testis/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q86W47 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0007268: chemical synaptic transmission |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0006813: potassium ion transport |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
enables |
GO:0015269: calcium-activated potassium channel activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q12791 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:KCMB4 |
involved_in |
GO:0019229: regulation of vasoconstriction |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0019228: neuronal action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
part_of |
GO:0008076: voltage-gated potassium channel complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
located_in |
GO:0005887: integral component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0006813: potassium ion transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0005513: detection of calcium ion |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0001508: action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB4 |
involved_in |
GO:0046928: regulation of neurotransmitter secretion |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
enables |
GO:0015269: calcium-activated potassium channel activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
involved_in |
GO:0019228: neuronal action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
part_of |
GO:0008076: voltage-gated potassium channel complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
located_in |
GO:0005887: integral component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
involved_in |
GO:0006813: potassium ion transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
involved_in |
GO:0005513: detection of calcium ion |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB3 |
involved_in |
GO:0001508: action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
enables |
GO:0015269: calcium-activated potassium channel activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
involved_in |
GO:0019229: regulation of vasoconstriction |
ECO:0000304: author statement supported by traceable reference used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
involved_in |
GO:0019228: neuronal action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
part_of |
GO:0008076: voltage-gated potassium channel complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
located_in |
GO:0005887: integral component of plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
involved_in |
GO:0006813: potassium ion transport |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
involved_in |
GO:0005513: detection of calcium ion |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KCMB2 |
involved_in |
GO:0001508: action potential |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
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