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PMID:10627043

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Citation

Panaretou, B, Sinclair, K, Prodromou, C, Johal, J, Pearl, L and Piper, PW (1999) The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections. Microbiology (Reading, Engl.) 145 ( Pt 12):3455-63

Abstract

During infections with a number of important eukaryotic pathogens the Hsp90 molecular chaperone of the pathogen is recognized as an immunodominant antigen by the host immune system. Yeast molecular genetics should allow study of the extent of sequence variation within conserved immunodominant epitopes on pathogen Hsp90s that is compatible with essential Hsp90 functions, as well as the processes that generate antigenic subfragments of these Hsp90s. The Hsp90 of the fungal pathogen Candida albicans was shown in this study to provide both essential and nonessential (pheromone signalling and mammalian steroid receptor activation) Hsp90 functions in Saccharomyces cerevisiae cells. Much of the C. albicans Hsp90 expressed in respiratory S. cerevisiae cells was shown to undergo a partial degradation in vivo, a degradation that closely resembles that of the native Hsp82 (one isoform of the homologous Hsp90) in S. cerevisiae. Allowing for the differences in the length of the charged linker region between the N- and C-terminal domains of C. albicans Hsp90 and S. cerevisiae Hsp82, these two proteins expressed in S. cerevisiae appear to give the same major degradation products. These Hsp90 fragments are similar to the products of incomplete Hsp90 degradation found in C. albicans cultures.

Links

PubMed

Keywords

Antigens, Fungal/immunology; Blotting, Western; Candida albicans/genetics; Candida albicans/metabolism; Candidiasis/immunology; Candidiasis/metabolism; HSP90 Heat-Shock Proteins/genetics; HSP90 Heat-Shock Proteins/immunology; HSP90 Heat-Shock Proteins/metabolism; Heat-Shock Proteins/metabolism; Heat-Shock Response; Peptide Fragments/immunology; Pheromones/metabolism; Receptors, Glucocorticoid/metabolism; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CANAL:HSP90

acts_upstream_of_or_within

GO:0030518: intracellular steroid hormone receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

P

Seeded From UniProt

complete

CANAL:HSP90

acts_upstream_of_or_within

GO:0006457: protein folding

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

P

Seeded From UniProt

complete

CANAL:HSP90

enables

GO:0016887: ATP hydrolysis activity

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

F

Seeded From UniProt

complete

CANAL:HSP90

enables

GO:0042623: ATPase activity, coupled

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

F

Seeded From UniProt

complete

CANAL:HSP90

involved_in

GO:0030518: intracellular steroid hormone receptor signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

P

Seeded From UniProt

complete

CANAL:HSP90

involved_in

GO:0006457: protein folding

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000004798
SGD:S000006161

P

Seeded From UniProt

complete

See also

References

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