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PMID:10624965

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Citation

Poliak, S, Gollan, L, Martinez, R, Custer, A, Einheber, S, Salzer, JL, Trimmer, JS, Shrager, P and Peles, E (1999) Caspr2, a new member of the neurexin superfamily, is localized at the juxtaparanodes of myelinated axons and associates with K+ channels. Neuron 24:1037-47

Abstract

Rapid conduction in myelinated axons depends on the generation of specialized subcellular domains to which different sets of ion channels are localized. Here, we describe the identification of Caspr2, a mammalian homolog of Drosophila Neurexin IV (Nrx-IV), and show that this neurexin-like protein and the closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region. We further show that Caspr2 specifically associates with Kv1.1, Kv1.2, and their Kvbeta2 subunit. This association involves the C-terminal sequence of Caspr2, which contains a putative PDZ binding site. These results suggest a role for Caspr family members in the local differentiation of the axon into distinct functional subdomains.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Axons/metabolism; Axons/ultrastructure; Blotting, Northern; Fluorescent Antibody Technique, Indirect; Humans; Immunohistochemistry; Kv1.1 Potassium Channel; Membrane Proteins/biosynthesis; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Microscopy, Electron; Molecular Sequence Data; Nerve Fibers, Myelinated/metabolism; Nerve Fibers, Myelinated/ultrastructure; Nerve Tissue Proteins/biosynthesis; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Nervous System/metabolism; Potassium Channels/metabolism; Potassium Channels, Voltage-Gated; Precipitin Tests; Rats

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:KCAB2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1307076

F

Seeded From UniProt

complete

RAT:KCNA2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:1307076

F

Seeded From UniProt

complete

RAT:KCNA2

located_in

GO:0044224: juxtaparanode region of axon

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNTP1

located_in

GO:0033270: paranode region of axon

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNTP1

involved_in

GO:0042552: myelination

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CNTP2

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

located_in

GO:0030425: dendrite

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

located_in

GO:0043025: neuronal cell body

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

involved_in

GO:0007420: brain development

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CNTP2

involved_in

GO:0008038: neuron recognition

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:CNTP2

part_of

GO:0008076: voltage-gated potassium channel complex

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

part_of

GO:0030425: dendrite

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

part_of

GO:0043025: neuronal cell body

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

part_of

GO:0008076: voltage-gated potassium channel complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:CNTP2

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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