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PMID:10619843

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Citation

de Bernard, M, Moschioni, M, Napolitani, G, Rappuoli, R and Montecucco, C (2000) The VacA toxin of Helicobacter pylori identifies a new intermediate filament-interacting protein. EMBO J. 19:48-56

Abstract

The VacA toxin produced by Helicobacter pylori acts inside cells and induces the formation of vacuoles arising from late endosomal/lysosomal compartments. Using VacA as bait in a yeast two-hybrid screening of a HeLa cell library, we have identified a novel protein of 54 kDa (VIP54), which interacts specifically with VacA, as indicated by co-immunoprecipitation and binding experiments. VIP54 is expressed in cultured cells and many tissues, with higher expression in the brain, muscle, kidney and liver. Confocal immunofluorescence microscopy with anti-VIP54 affinity- purified antibodies shows a fibrous pattern typical of intermediate filaments. Double label immunofluorescence performed on various cell lines with antibodies specific to different intermediate filament proteins revealed that VIP54 largely co-distributes with vimentin. In contrast to known intermediate filament proteins, VIP54 is predicted to contain approximately 50% of helical segments, but no extended coiled-coil regions. The possible involvement of this novel protein in interactions between intermediate filaments and late endosomal compartments is discussed.

Links

PubMed PMC1171776 Online version:10.1093/emboj/19.1.48

Keywords

Amino Acid Sequence; Animals; Astrocytes/chemistry; Bacterial Proteins/metabolism; Bacterial Toxins/metabolism; Cell Line; Cytotoxins/metabolism; Dogs; HeLa Cells; Helicobacter pylori/metabolism; Humans; Intermediate Filament Proteins/immunology; Intermediate Filament Proteins/isolation & purification; Intermediate Filament Proteins/metabolism; Mice; Microscopy, Fluorescence; Molecular Sequence Data; Molecular Weight; Neuroblastoma/chemistry; Neurons/chemistry; Tumor Cells, Cultured; Vacuoles/metabolism; Vimentin/isolation & purification

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SPN90

located_in

GO:0005882: intermediate filament

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SPN90

part_of

GO:0005882: intermediate filament

ECO:0000303: author statement without traceable support used in manual assertion

C

Seeded From UniProt

complete

HUMAN:SPN90

involved_in

GO:0007010: cytoskeleton organization

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

HUMAN:SPN90

enables

GO:0008092: cytoskeletal protein binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete


See also

References

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