PMID:10611233

Citation	Miller, ME and Cross, FR (2000) Distinct subcellular localization patterns contribute to functional specificity of the Cln2 and Cln3 cyclins of Saccharomyces cerevisiae. Mol. Cell. Biol. 20:542-55
Abstract	The G(1) cyclins of budding yeast drive cell cycle initiation by different mechanisms, but the molecular basis of their specificity is unknown. Here we test the hypothesis that the functional specificity of G(1) cyclins is due to differential subcellular localization. As shown by indirect immunofluorescence and biochemical fractionation, Cln3p localization appears to be primarily nuclear, with the most obvious accumulation of Cln3p to the nuclei of large budded cells. In contrast, Cln2p localizes to the cytoplasm. We were able to shift localization patterns of truncated Cln3p by the addition of nuclear localization and nuclear export signals, and we found that nuclear localization drives a Cln3p-like functional profile, while cytoplasmic localization leads to a partial shift to a Cln2p-like functional profile. Therefore, forcing Cln3p into a Cln2p-like cytoplasmic localization pattern partially alters the functional specificity of Cln3p toward that of Cln2p. These results suggest that there are CLN-dependent cytoplasmic and nuclear events important for cell cycle initiation. This is the first indication of a cytoplasmic function for a cyclin-dependent kinase. The data presented here support the idea that cyclin function is regulated at the level of subcellular localization and that subcellular localization contributes to the functional specificity of Cln2p and Cln3p.
Links	PubMed PMC85127
Keywords	Biological Transport; CDC28 Protein Kinase, S cerevisiae/metabolism; Cell Division; Cell Fractionation; Cell Nucleus/metabolism; Cyclins/chemistry; Cyclins/genetics; Cyclins/metabolism; Cytoplasm/metabolism; Fluorescent Antibody Technique, Indirect; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; Genes, Fungal/genetics; Genes, Fungal/physiology; Kinetics; Molecular Weight; Mutation/genetics; Nuclear Localization Signals; Phosphorylation; Precipitin Tests; Protein Binding; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Substrate Specificity
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