PMID:10559333

Citation	Timchenko, T, de Kouchkovsky, F, Katul, L, David, C, Vetten, HJ and Gronenborn, B (1999) A single rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants. J. Virol. 73:10173-82
Abstract	Faba bean necrotic yellows virus (FBNYV) belongs to the nanoviruses, plant viruses whose genome consists of multiple circular single-stranded DNA components. Eleven distinct DNAs, 5 of which encode different replication initiator (Rep) proteins, have been identified in two FBNYV isolates. Origin-specific DNA cleavage and nucleotidyl transfer activities were shown for Rep1 and Rep2 proteins in vitro, and their essential tyrosine residues that catalyze these reactions were identified by site-directed mutagenesis. In addition, we showed that Rep1 and Rep2 proteins hydrolyze ATP, and by changing the key lysine residue in the proteins' nucleoside triphosphate binding sites, demonstrated that this ATPase activity is essential for multiplication of virus DNA in vivo. Each of the five FBNYV Rep proteins initiated replication of the DNA molecule by which it was encoded, but only Rep2 was able to initiate replication of all the six other genome components. Furthermore, of the five rep components, only the Rep2-encoding DNA was always detected in 55 FBNYV samples from eight countries. These data provide experimental evidence for a master replication protein encoded by a multicomponent single-stranded DNA virus.
Links	PubMed PMC113070
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Base Sequence; DNA Helicases/genetics; DNA Helicases/metabolism; DNA Replication; DNA Viruses/genetics; DNA Viruses/metabolism; DNA, Single-Stranded; DNA, Viral/biosynthesis; DNA-Binding Proteins; Fabaceae/virology; Genome, Viral; Molecular Sequence Data; Plant Viruses/genetics; Plant Viruses/metabolism; Plants, Medicinal; RNA Nucleotidyltransferases/genetics; RNA Nucleotidyltransferases/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Trans-Activators/genetics; Trans-Activators/metabolism; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Replication
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
FBNY2:REP1	enables	GO:0016779: nucleotidyltransferase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
FBNY2:REP1	enables	GO:0016887: ATPase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
FBNY2:REP1	involved_in	GO:0019071: viral DNA cleavage involved in viral genome maturation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
FBNY2:REP1		GO:0016887: ATPase activity	ECO:0000314:		F		Figure 6	complete CACAO 1970
FBNY2:REP1		GO:0016779 : nucleotidyltransferase activity	ECO:0000314:		F		Figure 4	complete CACAO 1971
FBNY2:REP1		GO:0019071: viral DNA cleavage	ECO:0000314:		P		Figure:4B	complete CACAO 1977
FBNY2:MREP		GO:0016779: nucleotidyltransferase activity	ECO:0000314:		F		Figure 4	complete CACAO 2112
FBNY2:MREP	enables	GO:0016779: nucleotidyltransferase activity	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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