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PMID:10551833

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Citation

Yang, J and Kramer, JM (1999) Proteolytic processing of Caenorhabditis elegans SQT-1 cuticle collagen is inhibited in right roller mutants whereas cross-linking is inhibited in left roller mutants. J. Biol. Chem. 274:32744-9

Abstract

The sqt-1 gene encodes a C. elegans cuticle collagen that when defective can cause dramatic alterations of organismal morphology. Specific antisera were used to examine the assembly of wild-type and mutant SQT-1 in the cuticle. Wild-type SQT-1 chains associate into dimer, tetramer, and higher oligomers that are cross-linked by non-reducible, presumably tyrosine-derived, covalent bonds. The SQT-1 pattern differs from the bulk of cuticle collagens which are found in trimer and larger forms. sqt-1 mutations that cause left-handed helical twisting of animals remove a conserved carboxyl-domain cysteine and inhibit formation of these non-reducible bonds. SQT-1 monomers accumulate and novel trimer-sized products form. A conserved tyrosine immediately adjacent to the affected cysteine suggests that disulfide bond formation is required for this tyrosine to form a cross-link. sqt-1 mutations that cause right-handed helical twisting affect conserved arginines in a predicted cleavage site for a subtilisin-like protease. These mutant SQT-1 molecules retain residues on the amino side of the predicted cleavage site and are larger than wild-type by the amount expected if cleavage failed to occur. The conservation of this site in all nematode cuticle collagens indicates that they are all synthesized as procollagens that are processed by subtilisin-like proteases.

Links

PubMed

Keywords

Animals; Blotting, Western; Caenorhabditis elegans/genetics; Caenorhabditis elegans/metabolism; Collagen/chemistry; Collagen/genetics; Cross-Linking Reagents/metabolism; Cysteine/chemistry; Helminth Proteins/genetics; Helminth Proteins/metabolism; Mutation; Procollagen/metabolism; Protein Conformation; Subtilisins/antagonists & inhibitors; Subtilisins/metabolism; Tyrosine/chemistry

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:SQT1

enables

GO:0042329: structural constituent of collagen and cuticulin-based cuticle

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00143937

F

Seeded From UniProt

complete

CAEEL:SQT1

involved_in

GO:0040002: collagen and cuticulin-based cuticle development

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00143937

P

Seeded From UniProt

complete


See also

References

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