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PMID:10535731

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Citation

Tabara, H, Sarkissian, M, Kelly, WG, Fleenor, J, Grishok, A, Timmons, L, Fire, A and Mello, CC (1999) The rde-1 gene, RNA interference, and transposon silencing in C. elegans. Cell 99:123-32

Abstract

Double-stranded (ds) RNA can induce sequence-specific inhibition of gene function in several organisms. However, both the mechanism and the physiological role of the interference process remain mysterious. In order to study the interference process, we have selected C. elegans mutants resistant to dsRNA-mediated interference (RNAi). Two loci, rde-1 and rde-4, are defined by mutants strongly resistant to RNAi but with no obvious defects in growth or development. We show that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates. Interestingly, several, but not all, RNAi-deficient strains exhibit mobilization of the endogenous transposons. We discuss implications for the mechanism of RNAi and the possibility that one natural function of RNAi is transposon silencing.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Caenorhabditis elegans/genetics; Caenorhabditis elegans Proteins; Chromosomes, Artificial, Yeast; Cosmids; DNA Transposable Elements/genetics; Green Fluorescent Proteins; Helminth Proteins/biosynthesis; Helminth Proteins/chemistry; Helminth Proteins/genetics; Homozygote; Luminescent Proteins/genetics; Molecular Sequence Data; Mutation; RNA, Double-Stranded/genetics; RNA, Helminth/genetics; Sequence Alignment; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5EBF5

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090969
WB:WBVar00090971

P

Seeded From UniProt

complete

CAEEL:G5EEH0

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090963
WB:WBVar00090967
WB:WBVar00090970

P

Seeded From UniProt

complete

CAEEL:O44768

involved_in

GO:0000335: negative regulation of transposition, DNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete

CAEEL:O44768

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete

CAEEL:O44768

involved_in

GO:0045132: meiotic chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete

CAEEL:MUT7

involved_in

GO:0000335: negative regulation of transposition, DNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00239242

P

Seeded From UniProt

complete

CAEEL:MUT7

involved_in

GO:0016441: posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00239242

P

Seeded From UniProt

complete

CAEEL:MUT7

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00239242

P

Seeded From UniProt

complete

CAEEL:Q19672

involved_in

GO:0000335: negative regulation of transposition, DNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q19672

involved_in

GO:0016441: posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q19672

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q19672

involved_in

GO:0045132: meiotic chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q58AA4

involved_in

GO:0000335: negative regulation of transposition, DNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q58AA4

involved_in

GO:0016441: posttranscriptional gene silencing

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q58AA4

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q58AA4

involved_in

GO:0045132: meiotic chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090964

P

Seeded From UniProt

complete

CAEEL:Q8IA66

involved_in

GO:0000335: negative regulation of transposition, DNA-mediated

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete

CAEEL:Q8IA66

involved_in

GO:0016246: RNA interference

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete

CAEEL:Q8IA66

involved_in

GO:0045132: meiotic chromosome segregation

ECO:0000315: mutant phenotype evidence used in manual assertion

WB:WBVar00090968

P

Seeded From UniProt

complete


See also

References

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