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PMID:10502515

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Citation

Kivelä, HM, Männistö, RH, Kalkkinen, N and Bamford, DH (1999) Purification and protein composition of PM2, the first lipid-containing bacterial virus to be isolated. Virology 262:364-74

Abstract

The marine, icosahedral bacteriophage PM2 was isolated in the late 1960s. It was the first phage for which lipids were firmly demonstrated to be part of the virion structure and it has been classified as the type organism of the Corticoviridae family. The host, Pseudoalteromonas espejiana BAL-31, belongs to a common group of marine bacteria. We developed a purification method producing virions with specific infectivity approximately as high as that of the lipid-containing phages PRD1 and φ6. The sensitivity of the virus to normally used purification media such as those containing sucrose is demonstrated. We also present an alternative host, a pseudoalteromonad, that allows enhanced purification of the virus under reduced salt conditions. We show, using N-terminal amino acid sequencing and comparison with the genomic sequence, that there are at least eight structural proteins in the infectious virus.

Links

PubMed Online version:10.1006/viro.1999.9838

Keywords

Amino Acid Sequence; Bacteria/classification; Bacteria/virology; Calcium Chloride/pharmacology; Centrifugation, Zonal; Cesium; Chlorides; Corticoviridae/chemistry; Corticoviridae/drug effects; Corticoviridae/growth & development; Corticoviridae/isolation & purification; Genome, Viral; Glycerol; Immune Sera/pharmacology; Lipids/analysis; Molecular Sequence Data; Molecular Weight; RNA, Ribosomal, 16S/analysis; RNA, Viral/analysis; Sequence Analysis; Sodium Chloride/pharmacology; Sucrose; Time Factors; Triiodobenzoic Acids; Viral Structural Proteins/chemistry; Viral Structural Proteins/genetics; Viral Structural Proteins/isolation & purification

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPPM2:CAPSD

GO:0098017: viral capsid, major subunit

ECO:0000314:

C

Figure 5.

complete
CACAO 6264

BPPM2:CAPSD

part_of

GO:0098017: viral capsid, major subunit

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:SPIKE

GO:0019012: virion

ECO:0000314:

C

Figure 5.

complete
CACAO 5805

BPPM2:SPIKE

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P3

GO:0019012: virion

ECO:0000314:

C

Figure 5.

complete
CACAO 5807

BPPM2:P3

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P4

GO:0019012: virion

ECO:0000314:

C

Figure 5.

complete
CACAO 5808

BPPM2:P4

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P5

GO:0019012: virion membrane

ECO:0000314:

C

Figure 5.

complete
CACAO 5809

BPPM2:P5

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P6

GO:0019012: virion membrane

ECO:0000314:

C

Figure 5.

complete
CACAO 5810

BPPM2:P6

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P7

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:P8

GO:0019012: virion membrane

ECO:0000314:

C

Figure 5.

complete
CACAO 5812

BPPM2:P8

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPM2:EXLYS

GO:0019012: virion membrane

ECO:0000314:

C

Figure 5.

complete
CACAO 5811

BPPM2:EXLYS

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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