PMID:10498729

Citation	Price, B, Adamidis, T, Kong, R and Champness, W (1999) A Streptomyces coelicolor antibiotic regulatory gene, absB, encodes an RNase III homolog. J. Bacteriol. 181:6142-51
Abstract	Streptomyces coelicolor produces four genetically and structurally distinct antibiotics in a growth-phase-dependent manner. S. coelicolor mutants globally deficient in antibiotic production (Abs(-) phenotype) have previously been isolated, and some of these were found to define the absB locus. In this study, we isolated absB-complementing DNA and show that it encodes the S. coelicolor homolog of RNase III (rnc). Several lines of evidence indicate that the absB mutant global defect in antibiotic synthesis is due to a deficiency in RNase III. In marker exchange experiments, the S. coelicolor rnc gene rescued absB mutants, restoring antibiotic production. Sequencing the DNA of absB mutants confirmed that the absB mutations lay in the rnc open reading frame. Constructed disruptions of rnc in both S. coelicolor 1501 and Streptomyces lividans 1326 caused an Abs(-) phenotype. An absB mutation caused accumulation of 30S rRNA precursors, as had previously been reported for E. coli rnc mutants. The absB gene is widely conserved in streptomycetes. We speculate on why an RNase III deficiency could globally affect the synthesis of antibiotics.
Links	PubMed PMC103644
Keywords	Amino Acid Sequence; Anti-Bacterial Agents/biosynthesis; Base Sequence; Cloning, Molecular; Endoribonucleases/genetics; Genes, Bacterial; Genes, Regulator; Genetic Complementation Test; Molecular Sequence Data; Restriction Mapping; Ribonuclease III; Sequence Homology, Amino Acid; Species Specificity; Streptomyces/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STRCO:RNC		GO:0017000: antibiotic biosynthetic process	ECO:0000314:		P		Figure 2. shows the PCR primers A and D in the experiment. They were used to make a absB gene template from the wild-type and mutant strains (shown in table 1). The primers were inserted into an E. coli replicon, pBluescript SK(+) (pBK802 to pBK804 and pRK805), in order to be sequences with normal primers and reversed primers. The results of the experiment show that the abs gene encodes the S. coelicolor homolog of RNase III. Also, through a series of processes, absB mutant can be transformed in the wild-type Abs+ when using a gene homologous to the wild-type RNAse III.	complete CACAO 13183

References

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