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PMID:10498613

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Citation

Aguiar, RC, Yakushijin, Y, Kharbanda, S, Tiwari, S, Freeman, GJ and Shipp, MA (1999) PTPROt: an alternatively spliced and developmentally regulated B-lymphoid phosphatase that promotes G0/G1 arrest. Blood 94:2403-13

Abstract

Protein tyrosine phosphatases (PTP) regulate the proliferation, differentiation, and viability of lymphocytes by modulating their signaling pathways. By using the differential display assay, we have cloned a putative receptor-type PTP, which is predominantly expressed in B-lymphoid tissues (lymph nodes and spleen). This PTP, termed PTPROt (truncated), is a tissue-specific alternatively-spliced form of a human epithelial PTP, PTPRO (PTPU2/GLEPP1). Whereas the epithelial PTPRO includes an approximately 800-amino acid extracellular domain, the major (3 kb) PTPROt cDNA predicts a unique 5' untranslated region and truncated (8 amino acids) extracellular domain with a conserved transmembrane region and single catalytic domain. PTPROt cDNAs encode functional approximately 47-kD and approximately 43-kD PTPs, which are most abundant in normal naive quiescent B cells and decreased or absent in germinal center B cells and germinal center-derived diffuse large B-cell lymphomas. Because PTPROt was predominantly expressed in naive quiescent B cells, the enzyme's effects on cell-cycle progression were examined. When multiple stable PTPROt sense, antisense, and vector only B-cell transfectants were grown in reduced serum and synchronized with nocodazole, PTPROt sense clones exhibited markedly increased G0/G1 arrest. Taken together, these data implicate PTPROt in the growth control of specific B-cell subpopulations.

Links

PubMed

Keywords

Alternative Splicing; Amino Acid Sequence; B-Lymphocytes/cytology; B-Lymphocytes/enzymology; Base Sequence; Cell Cycle/drug effects; Cell Cycle/physiology; Cloning, Molecular; Conserved Sequence; DNA, Complementary; G1 Phase; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Humans; Introns; Lymphoma, B-Cell/enzymology; Lymphoma, B-Cell/genetics; Molecular Sequence Data; Nocodazole/pharmacology; Palatine Tonsil/immunology; Protein Tyrosine Phosphatases/chemistry; Protein Tyrosine Phosphatases/genetics; Protein Tyrosine Phosphatases/metabolism; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Resting Phase, Cell Cycle; Sequence Deletion; Spleen/immunology; Transfection; Tumor Cells, Cultured

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PTPRO

located_in

GO:0016021: integral component of membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PTPRO

part_of

GO:0016021: integral component of membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:PTPRO

involved_in

GO:0006470: protein dephosphorylation

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PTPRO

enables

GO:0004725: protein tyrosine phosphatase activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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