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PMID:10497209
| Citation |
Almenar-Queralt, A, Lee, A, Conley, CA, Ribas de Pouplana, L and Fowler, VM (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle. J. Biol. Chem. 274:28466-75 |
|---|---|
| Abstract |
Tropomodulin (E-Tmod) is an actin filament pointed end capping protein that maintains the length of the sarcomeric actin filaments in striated muscle. Here, we describe the identification and characterization of a novel tropomodulin isoform, skeletal tropomodulin (Sk-Tmod) from chickens. Sk-Tmod is 62% identical in amino acid sequence to the previously described chicken E-Tmod and is the product of a different gene. Sk-Tmod isoform sequences are highly conserved across vertebrates and constitute an independent group in the tropomodulin family. In vitro, chicken Sk-Tmod caps actin and tropomyosin-actin filament pointed ends to the same extent as does chicken E-Tmod. However, E- and Sk-Tmods differ in their tissue distribution; Sk-Tmod predominates in fast skeletal muscle fibers, lens, and erythrocytes, while E-Tmod is found in heart and slow skeletal muscle fibers. Additionally, their expression is developmentally regulated during chicken breast muscle differentiation with Sk-Tmod replacing E-Tmod after hatching. Finally, in skeletal muscle fibers that coexpress both Sk- and E-Tmod, they are recruited to different actin filament-containing cytoskeletal structures within the cell: myofibrils and costameres, respectively. All together, these observations support the hypothesis that vertebrates have acquired different tropomodulin isoforms that play distinct roles in vivo. |
| Links | |
| Keywords |
Actins/metabolism; Amino Acid Sequence; Animals; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chick Embryo; Chickens; DNA, Complementary; Microfilament Proteins; Molecular Sequence Data; Muscle Development; Muscle, Skeletal/growth & development; Muscle, Skeletal/metabolism; Protein Isoforms/genetics; Protein Isoforms/metabolism; Sequence Homology, Amino Acid; Spectrin/metabolism; Tropomodulin |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
CHICK:F1NHT3 |
located_in |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
CHICK:F1NY71 |
located_in |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
CHICK:F1NY71 |
located_in |
GO:0042383: sarcolemma |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
CHICK:H9KZU7 |
located_in |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
CHICK:H9KZU7 |
located_in |
GO:0005865: striated muscle thin filament |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
CHICK:H9KZU7 |
involved_in |
GO:0051694: pointed-end actin filament capping |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
CHICK:H9KZU7 |
enables |
GO:0051015: actin filament binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
CHICK:SPTN1 |
located_in |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | |
|
located_in |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0042383: sarcolemma |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0005865: striated muscle thin filament |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0042383: sarcolemma |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
located_in |
GO:0005865: striated muscle thin filament |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(CL:0008002) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0042383: sarcolemma |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(CL:0008002) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(CL:0000232) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005623: cell |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
enables |
GO:0051015: actin filament binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051694: pointed-end actin filament capping |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005865: striated muscle thin filament |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(UBERON:0001495) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0043034: costamere |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(CL:0008002) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0042383: sarcolemma |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0051694: pointed-end actin filament capping |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005623: cell |
ECO:0000314: direct assay evidence used in manual assertion |
C |
|
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005865: striated muscle thin filament |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(UBERON:0001495) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0016020: membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
part_of:(CL:0000232) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0051015: actin filament binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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