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PMID:10497209

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Citation

Almenar-Queralt, A, Lee, A, Conley, CA, Ribas de Pouplana, L and Fowler, VM (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle. J. Biol. Chem. 274:28466-75

Abstract

Tropomodulin (E-Tmod) is an actin filament pointed end capping protein that maintains the length of the sarcomeric actin filaments in striated muscle. Here, we describe the identification and characterization of a novel tropomodulin isoform, skeletal tropomodulin (Sk-Tmod) from chickens. Sk-Tmod is 62% identical in amino acid sequence to the previously described chicken E-Tmod and is the product of a different gene. Sk-Tmod isoform sequences are highly conserved across vertebrates and constitute an independent group in the tropomodulin family. In vitro, chicken Sk-Tmod caps actin and tropomyosin-actin filament pointed ends to the same extent as does chicken E-Tmod. However, E- and Sk-Tmods differ in their tissue distribution; Sk-Tmod predominates in fast skeletal muscle fibers, lens, and erythrocytes, while E-Tmod is found in heart and slow skeletal muscle fibers. Additionally, their expression is developmentally regulated during chicken breast muscle differentiation with Sk-Tmod replacing E-Tmod after hatching. Finally, in skeletal muscle fibers that coexpress both Sk- and E-Tmod, they are recruited to different actin filament-containing cytoskeletal structures within the cell: myofibrils and costameres, respectively. All together, these observations support the hypothesis that vertebrates have acquired different tropomodulin isoforms that play distinct roles in vivo.

Links

PubMed

Keywords

Actins/metabolism; Amino Acid Sequence; Animals; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/metabolism; Chick Embryo; Chickens; DNA, Complementary; Microfilament Proteins; Molecular Sequence Data; Muscle Development; Muscle, Skeletal/growth & development; Muscle, Skeletal/metabolism; Protein Isoforms/genetics; Protein Isoforms/metabolism; Sequence Homology, Amino Acid; Spectrin/metabolism; Tropomodulin

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:F1NHT3

located_in

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:F1NY71

located_in

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:F1NY71

located_in

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)|part_of:(UBERON:0006907)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:H9KZU7

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000232)

Seeded From UniProt

complete

CHICK:H9KZU7

located_in

GO:0005865: striated muscle thin filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)

Seeded From UniProt

complete

CHICK:H9KZU7

involved_in

GO:0051694: pointed-end actin filament capping

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:H9KZU7

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:SPTN1

located_in

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q91006

located_in

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q91006

located_in

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)|part_of:(UBERON:0006907)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q9DEA4

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000232)

Seeded From UniProt

complete

CHICK:Q9DEA4

located_in

GO:0005865: striated muscle thin filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)

Seeded From UniProt

complete

CHICK:Q9DEA6

located_in

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q9DEA6

located_in

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)|part_of:(UBERON:0006907)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q9PUQ7

located_in

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000232)

Seeded From UniProt

complete

CHICK:Q9PUQ7

located_in

GO:0005865: striated muscle thin filament

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)

Seeded From UniProt

complete

CHICK:SPTA2

part_of

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q91006

part_of

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)|part_of:(UBERON:0006907)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q91006

part_of

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q9DEA4

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000232)

Seeded From UniProt

complete

CHICK:Q9DEA4

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0000965)|part_of:(UBERON:0006908)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q9DEA4

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CHICK:Q9DEA4

involved_in

GO:0051694: pointed-end actin filament capping

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q9DEA4

part_of

GO:0005865: striated muscle thin filament

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0001495)

Seeded From UniProt

complete

CHICK:Q9DEA6

part_of

GO:0043034: costamere

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0008002)

Seeded From UniProt

complete

CHICK:Q9DEA6

part_of

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001495)|part_of:(UBERON:0006907)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q9PUQ7

involved_in

GO:0051694: pointed-end actin filament capping

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

CHICK:Q9PUQ7

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0000965)|part_of:(UBERON:0006908)
  • part_of:(UBERON:0001374)

Seeded From UniProt

complete

CHICK:Q9PUQ7

part_of

GO:0005865: striated muscle thin filament

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(UBERON:0001495)

Seeded From UniProt

complete

CHICK:Q9PUQ7

part_of

GO:0016020: membrane

ECO:0000314: direct assay evidence used in manual assertion

C

part_of:(CL:0000232)

Seeded From UniProt

complete

CHICK:Q9PUQ7

enables

GO:0051015: actin filament binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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