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PMID:10477523

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Citation

Kleiman, FE and Manley, JL (1999) Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50. Science 285:1576-9

Abstract

Polyadenylation of messenger RNA precursors requires a complex protein machinery that is closely integrated with the even more complex transcriptional apparatus. Here a polyadenylation factor, CstF-50 (cleavage stimulation factor), is shown to interact in vitro and in intact cells with a nuclear protein of previously unknown function, BRCA1-associated RING domain protein (BARD1). The BARD1-CstF-50 interaction inhibits polyadenylation in vitro. BARD1, like CstF-50, also interacts with RNA polymerase II. These results indicate that BARD1-mediated inhibition of polyadenylation may prevent inappropriate RNA processing during transcription, perhaps at sites of DNA repair, and they reveal an unanticipated integration of diverse nuclear events.

Links

PubMed

Keywords

Antibodies, Monoclonal; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Nucleus/metabolism; DNA Damage; DNA Repair; DNA-Binding Proteins/metabolism; HeLa Cells; Humans; Poly A/metabolism; Proliferating Cell Nuclear Antigen/metabolism; RNA Polymerase II/metabolism; RNA Precursors/metabolism; RNA, Messenger/metabolism; RNA-Binding Proteins/chemistry; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Rad51 Recombinase; Recombinant Fusion Proteins/metabolism; Repetitive Sequences, Amino Acid; Tumor Suppressor Proteins; Ubiquitin-Protein Ligases; Zinc Fingers; mRNA Cleavage and Polyadenylation Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:CSTF2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99728

F

Seeded From UniProt

complete

HUMAN:BRCA1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99728

F

Seeded From UniProt

complete

HUMAN:CSTF1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q99728

F

Seeded From UniProt

complete

HUMAN:BARD1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P33240

F

Seeded From UniProt

complete

HUMAN:BARD1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P38398

F

Seeded From UniProt

complete

HUMAN:BARD1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q05048

F

Seeded From UniProt

complete


See also

References

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