PMID:10465788

Citation	Yang, WC, Ye, D, Xu, J and Sundaresan, V (1999) The SPOROCYTELESS gene of Arabidopsis is required for initiation of sporogenesis and encodes a novel nuclear protein. Genes Dev. 13:2108-17
Abstract	The formation of haploid spores marks the initiation of the gametophytic phase of the life cycle of all vascular plants ranging from ferns to angiosperms. In angiosperms, this process is initiated by the differentiation of a subset of floral cells into sporocytes, which then undergo meiotic divisions to form microspores and megaspores. Currently, there is little information available regarding the genes and proteins that regulate this key step in plant reproduction. We report here the identification of a mutation, SPOROCYTELESS (SPL), which blocks sporocyte formation in Arabidopsis thaliana. Analysis of the SPL mutation suggests that development of the anther walls and the tapetum and microsporocyte formation are tightly coupled, and that nucellar development may be dependent on megasporocyte formation. Molecular cloning of the SPL gene showed that it encodes a novel nuclear protein related to MADS box transcription factors and that it is expressed during microsporogenesis and megasporogenesis. These data suggest that the SPL gene product is a transcriptional regulator of sporocyte development in Arabidopsis.
Links	PubMed PMC316961
Keywords	Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/growth & development; Arabidopsis Proteins; Base Sequence; DNA, Plant; Genes, Plant; Humans; Molecular Sequence Data; Mutation; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; Plant Proteins/genetics; Plant Proteins/physiology; Repressor Proteins; Sequence Homology, Amino Acid; Spores; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription Factors/physiology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:SPL	located_in	GO:0005634: nucleus	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
ARATH:SPL	acts_upstream_of_or_within	GO:0048533: sporocyte differentiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:SPL	enables	GO:0003700: DNA-binding transcription factor activity	ECO:0000250: sequence similarity evidence used in manual assertion		F		Seeded From UniProt	complete
ARATH:SPL	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	AGI_LocusCode:AT2G45190	F		Seeded From UniProt	complete

References

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