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PMID:10457025

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Citation

Willert, K, Logan, CY, Arora, A, Fish, M and Nusse, R (1999) A Drosophila Axin homolog, Daxin, inhibits Wnt signaling. Development 126:4165-73

Abstract

The vertebrate Axin protein, the product of the mouse fused gene, binds to beta-catenin to inhibit Wnt signaling. We have identified a homolog of Axin in Drosophila, Daxin. Using double-stranded RNA interference, we generated loss-of-function phenotypes that are similar to overexpression of the Drosophila Wnt gene wingless (wg). Overexpression of Daxin produces phenotypes similar to loss of wg. In addition, we show that Daxin overexpression can modify phenotypes elicited by wg and another Drosophila Wnt gene, DWnt-2. Using immunoprecipitation of endogenous Daxin protein from embryos we show that Daxin interacts with Armadillo and Zeste-white 3. The loss-of-function and overexpression phenotypes show that Daxin, like its mammalian counterpart, acts as a negative regulator of wg/Wnt signaling.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Armadillo Domain Proteins; Axin Protein; Base Sequence; Carrier Proteins/genetics; Carrier Proteins/metabolism; Drosophila Proteins; Drosophila melanogaster/embryology; Drosophila melanogaster/genetics; Embryo, Nonmammalian; Expressed Sequence Tags; Eye/growth & development; Eye Abnormalities/genetics; Female; Gene Expression Regulation, Developmental; Genetic Techniques; Glycogen Synthase Kinase 3; Insect Proteins/genetics; Insect Proteins/metabolism; Molecular Sequence Data; Ovary/growth & development; Ovary/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Proteins/genetics; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins/metabolism; RNA/genetics; Repressor Proteins; Sequence Homology, Amino Acid; Signal Transduction; Trans-Activators; Transcription Factors; Wing/growth & development; Wnt1 Protein; Wnt2 Protein

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:AXN

involved_in

GO:0008587: imaginal disc-derived wing margin morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:AXN

involved_in

GO:0007367: segment polarity determination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:AXN

involved_in

GO:0035293: chitin-based larval cuticle pattern formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:AXN

involved_in

GO:0090090: negative regulation of canonical Wnt signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:AXN

involved_in

GO:0090090: negative regulation of canonical Wnt signaling pathway

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0004360

P

Seeded From UniProt

complete


See also

References

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