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PMID:10446428

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Citation

Ganesh Bhat, B, Wang, P, Kim, JH, Black, TM, Lewin, TM, Fiedorek, FT Jr and Coleman, RA (1999) Rat sn-glycerol-3-phosphate acyltransferase: molecular cloning and characterization of the cDNA and expressed protein. Biochim. Biophys. Acta 1439:415-23

Abstract

Rat mitochondrial glycerol-3-phosphate acyltransferase (GPAT) cDNA was cloned and characterized. We identified a cDNA containing an open reading frame of 828 amino acids that had an 89% homology with the coding region of the previously characterized mouse mitochondrial GPAT and a predicted amino acid sequence that was 96% identical. The rat 5' UTR was only 159 nucleotides, in contrast to the 926 nucleotide 5' UTR of the mouse cDNA and had an internal deletion of 167 nucleotides. GPAT was expressed in Sf21 insect cells, and specific inhibitors strongly suggest that, like the Escherichia coli GPAT, the recombinant mitochondrial GPAT and the mitochondrial GPAT isoform in rat liver contain critical serine, histidine, and arginine residues.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; DNA, Complementary/chemistry; DNA, Complementary/genetics; Glycerol-3-Phosphate O-Acyltransferase/biosynthesis; Glycerol-3-Phosphate O-Acyltransferase/chemistry; Glycerol-3-Phosphate O-Acyltransferase/genetics; Insects; Mitochondria, Liver/enzymology; Molecular Sequence Data; Rats; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:GPAT1

located_in

GO:0005739: mitochondrion

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

RAT:GPAT1

enables

GO:0004366: glycerol-3-phosphate O-acyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

RAT:GPAT1

part_of

GO:0005739: mitochondrion

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete


See also

References

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