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PMID:10438528

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Citation

Holmes, M, Turner, J, Fox, A, Chisholm, O, Crossley, M and Chong, B (1999) hFOG-2, a novel zinc finger protein, binds the co-repressor mCtBP2 and modulates GATA-mediated activation. J. Biol. Chem. 274:23491-8

Abstract

We have identified a novel human zinc finger protein, hFOG-2, which is related to but distinct from the murine transcription factor Friend-of-GATA-1 (mFOG-1). The hFOG-2 gene was initially detected in K562 cells using a polymerase chain reaction approach with degenerate primers corresponding to zinc finger regions of mFOG-1. A murine homologue of hFOG-2 was also identified in the mouse expressed sequence tag data banks, indicating that a family of FOG genes exists in mammals. hFOG-2 appears to be widely expressed, while mFOG-1 is expressed primarily in erythroid and megakaryocytic cells and plays a fundamental role in the development of these lineages. Sequencing of the full-length hFOG-2 cDNA indicates that the interaction domains for transcription factors GATA-1 and mCtBP2 are both conserved and we have shown that this new FOG protein also physically interacts with these factors. We have demonstrated that hFOG-2, like mFOG-1, can act in concert with GATA-1 to activate gene expression from the p45 NF-E2 promoter region, but that it can also act to repress GATA-mediated activation of additional reporter constructs. Finally, we have identified a repression domain in hFOG-2 and show that repression is dependent upon the integrity of the mCtBP2 interaction motif Pro-Ile-Asp-Leu-Ser.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cell Line; Cloning, Molecular; DNA Primers; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Erythroid-Specific DNA-Binding Factors; GATA1 Transcription Factor; Humans; Mice; Molecular Sequence Data; NF-E2 Transcription Factor; NF-E2 Transcription Factor, p45 Subunit; Phosphoproteins/metabolism; Promoter Regions, Genetic; Protein Binding; Repressor Proteins/metabolism; Sequence Homology, Amino Acid; Transcription Factors/metabolism; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:GATA1

enables

GO:0001221: transcription coregulator binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8WW38

F

Seeded From UniProt

complete

MOUSE:CTBP2

enables

GO:0001221: transcription coregulator binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q8WW38

F

Seeded From UniProt

complete

HUMAN:FOG2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P56546

F

Seeded From UniProt

complete

HUMAN:FOG2

involved_in

GO:0000122: negative regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FOG2

enables

GO:0008270: zinc ion binding

ECO:0000303: author statement without traceable support used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FOG2

enables

GO:0003713: transcription coactivator activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FOG2

enables

GO:0003714: transcription corepressor activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:FOG2

involved_in

GO:0045944: positive regulation of transcription by RNA polymerase II

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:FOG2

enables

GO:0061629: RNA polymerase II-specific DNA-binding transcription factor binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P17679

F

Seeded From UniProt

complete


See also

References

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