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PMID:10409118

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Citation

Flück, M, Carson, JA, Gordon, SE, Ziemiecki, A and Booth, FW (1999) Focal adhesion proteins FAK and paxillin increase in hypertrophied skeletal muscle. Am. J. Physiol. 277:C152-62

Abstract

Components of signaling pathways for mechanotransduction during load-induced enlargement of skeletal muscle have not been completely defined. We hypothesized that loading of skeletal muscle would result in an adaptive increase in the expression of two focal adhesion complex (FAC)-related proteins, focal adhesion kinase (FAK) and paxillin, as well as increased FAK activity. FAK protein was immunolocalized to the sarcolemmal region of rooster anterior latissimus dorsi (ALD) myofibers in the middle of the ALD muscle. FAK (77 and 81%) and paxillin (206 and 202%) protein concentrations per unit of total protein in Western blots increased significantly after 1.5 and 7 days, but not after 13 days, of stretch-induced hypertrophy-hyperplasia of the ALD muscle. FAK autokinase activity in immunoprecipitates was increased after 1.5, 7, and 13 days in stretched ALD muscles. To determine whether increased FAK and paxillin protein concentrations are associated with hypertrophy and/or new fiber formation, two additional experiments were performed. First, during formation of primary chicken myotubes (a model of new fiber formation), FAK protein concentration (63%), FAK activity (157%), and paxillin protein concentration (97%) increased compared with myoblasts. Second, FAK (112% and 611%) and paxillin (87% and 431%) protein concentrations per unit of total protein in the soleus muscle increased at 1 and 8 days after surgical ablation of the synergistic gastrocnemius muscle (a model of hypertrophy without hyperplasia). Thus increases in components of the FAC occur in hypertrophying muscle of animals and in newly formed muscle fibers in culture. Furthermore, increased FAK activity suggests a possible convergence of signaling at the FAC in load-induced growth of skeletal muscle.

Links

PubMed Online version:10.1152/ajpcell.1999.277.1.C152

Keywords

Animals; Cell Adhesion Molecules/metabolism; Chickens; Cytoskeletal Proteins/metabolism; Female; Focal Adhesion Kinase 1; Focal Adhesion Protein-Tyrosine Kinases; Hypertrophy/metabolism; Male; Muscle, Skeletal/metabolism; Muscle, Skeletal/pathology; Osmolar Concentration; Paxillin; Phosphoproteins/metabolism; Physical Stimulation; Protein-Tyrosine Kinases/metabolism; Rats; Rats, Sprague-Dawley; Tissue Distribution/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CHICK:F1NB68

involved_in

GO:0035994: response to muscle stretch

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001112)

Seeded From UniProt

complete

CHICK:VINC

located_in

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

CHICK:PAXI

involved_in

GO:0035994: response to muscle stretch

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001112)

Seeded From UniProt

complete

CHICK:FAK1

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

CHICK:FAK1

located_in

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

CHICK:FAK1

part_of

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

CHICK:FAK1

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

CHICK:FAK1

involved_in

GO:0046777: protein autophosphorylation

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001112)
  • occurs_in:(CL:0000187)|occurs_in:(CL:0002372)
  • occurs_in:(CL:0000515)

Seeded From UniProt

complete

CHICK:FAK1

involved_in

GO:0035994: response to muscle stretch

ECO:0000314: direct assay evidence used in manual assertion

P

  • occurs_in:(UBERON:0001112)
  • occurs_in:(CL:0000187)

Seeded From UniProt

complete

CHICK:PAXI

involved_in

GO:0035994: response to muscle stretch

ECO:0000314: direct assay evidence used in manual assertion

P

occurs_in:(UBERON:0001112)

Seeded From UniProt

complete

CHICK:PAXI

part_of

GO:0005623: cell

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0002372)
  • part_of:(CL:0000515)

Seeded From UniProt

complete

CHICK:VINC

part_of

GO:0042383: sarcolemma

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(UBERON:0001112)
  • part_of:(CL:0000187)

Seeded From UniProt

complete

Notes

See also

References

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