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PMID:10382967

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Citation

Miyake, T, Shiba, T, Kameda, A, Ihara, Y, Munekata, M, Ishige, K and Noguchi, T (1999) The gene for an exopolyphosphatase of Pseudomonas aeruginosa. DNA Res. 6:103-8

Abstract

In Pseudomonas aeriginosa, a gene, ppx, that encodes exopolyphosphatase [exopoly(P)ase; EC 3.6.1.11] of 506 amino acids (56,419 Da) was found downstream of the gene for polyphosphate kinase, ppk. Since ppx is located in the opposite direction of the ppk gene, they do not constitute an operon. The predicted amino acid sequence of PPX is 41% identical with Escherichia coli PPX. The gene product of ppx (paPPX) was overproduced in E. coli, and its activity was evaluated. Orthophosphate (Pi) is released from polyphosphate [poly(P)], the average chain lengths of which are 79 and 750, respectively. The amount of Pi released matched the amount of poly(P) lost. Thus ppx encodes an enzyme that has exopoly(P)ase activity.

Links

PubMed

Keywords

Acid Anhydride Hydrolases/genetics; Amino Acid Sequence; Base Sequence; DNA, Bacterial; Molecular Sequence Data; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:PPX

enables

GO:0004309: exopolyphosphatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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