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PMID:10380802

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Citation

Nuccio, ML and Thomas, TL (1999) ATS1 and ATS3: two novel embryo-specific genes in Arabidopsis thaliana. Plant Mol. Biol. 39:1153-63

Abstract

A modified protocol for differential display of mRNA was used to identify and clone genes expressed in developing Arabidopsis thaliana seeds. Two novel embryo-specific genes designated ATS1 and ATS3 (Arabidopsis thaliana seed gene) were identified. In situ hybridization showed that, spatially, ATS1 is expressed in a pattern similar to the Arabidopsis GEA1 gene and that ATS3 is expressed in a pattern similar to the Arabidopsis seed storage protein genes. Southern analysis of Arabidopsis genomic DNA indicated that ATS1 is a member of a small gene family and that ATS3 is present as a single copy in the diploid genome. Sequence analysis of both genes showed that ATS1 is similar to the rice EFA27 gene and that ATS3 is unique. Western analysis and light level immunocytochemistry using antisera raised against the putative ATS1 and ATS3 translation products verified that ATS1 and ATS3 proteins are seed-specific and accumulate in a spatial pattern similar to their respective transcripts. Taken together, these data show that ATS1 and ATS3 are novel embryo-specific genes in Arabidopsis.

Links

PubMed

Keywords

Arabidopsis/embryology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins; Blotting, Southern; Blotting, Western; Chromosomes/genetics; Cloning, Molecular; Gene Dosage; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Gene Library; Genes, Plant/genetics; Genome, Plant; Immunohistochemistry; In Situ Hybridization; Molecular Sequence Data; Open Reading Frames/genetics; Plant Proteins/chemistry; Plant Proteins/genetics; Plant Proteins/metabolism; RNA, Messenger/analysis; RNA, Messenger/genetics; RNA, Messenger/metabolism; Seeds/genetics; Seeds/growth & development; Seeds/metabolism; Sequence Homology, Nucleic Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:PXG1

acts_upstream_of_or_within

GO:0009793: embryo development ending in seed dormancy

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

ARATH:PXG1

NOT|involved_in

GO:0009737: response to abscisic acid

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATS3

acts_upstream_of_or_within

GO:0009793: embryo development ending in seed dormancy

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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