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PMID:10373452

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Citation

Okamoto, M, Schoch, S and Südhof, TC (1999) EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis? J. Biol. Chem. 274:18446-54

Abstract

In yeast two-hybrid screens for proteins that bind to SNAP-25 and may be involved in exocytosis, we isolated a protein called EHSH1 (for EH domain/SH3 domain-containing protein). Cloning of full-length cDNAs revealed that EHSH1 is composed of an N-terminal region with two EH domains, a central region that is enriched in lysine, leucine, glutamate, arginine, and glutamine (KLERQ domain), and a C-terminal region comprised of five SH3 domains. The third SH3 domain is alternatively spliced. Data bank searches demonstrated that EHSH1 is very similar to Xenopus and human intersectins and to human SH3P17. In addition, we identified expressed sequence tags that encode a second isoform of EHSH1, called EHSH2. EHSH1 is abundantly expressed in brain and at lower levels in all other tissues tested. In binding studies, we found that the central KLERQ domain of EHSH1 binds to recombinant or native brain SNAP-25 and SNAP-23. The C-terminal SH3 domains, by contrast, quantitatively interact with dynamin, a protein involved in endocytosis. Dynamin strongly binds to the alternatively spliced central SH3 domain (SH3C) and the two C-terminal SH3 domains (SH3D and SH3E) but not to the N-terminal SH3 domains (SH3A and SH3B). Immunoprecipitations confirmed that both dynamin and SNAP-25 are complexed to EHSH1 in brain. Our data suggest that EHSH1/intersectin may be a novel adaptor protein that couples endocytic membrane traffic to exocytosis. The ability of multiple SH3 domains in EHSH1 to bind to dynamin suggests that EHSH1 can cluster several dynamin molecules in a manner that is regulated by alternative splicing.

Links

PubMed

Keywords

Adaptor Proteins, Vesicular Transport; Amino Acid Sequence; Animals; Carrier Proteins/chemistry; Carrier Proteins/metabolism; Drosophila; Drosophila Proteins; Dynamins; Endocytosis; Exocytosis; GTP Phosphohydrolases/metabolism; Gene Library; Humans; Membrane Proteins; Mice; Molecular Sequence Data; Nerve Tissue Proteins/metabolism; Protein Binding; Rats; Synaptosomal-Associated Protein 25; Xenopus; src Homology Domains

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:SNP23

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2935

F

Seeded From UniProt

complete

RAT:DYN1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2935

F

Seeded From UniProt

complete

RAT:SNP25

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:2935

F

Seeded From UniProt

complete

RAT:ITSN1

involved_in

GO:0007420: brain development

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:ITSN1

located_in

GO:0045202: synapse

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:ITSN1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:3728
RGD:620221
RGD:71096

F

Seeded From UniProt

complete


See also

References

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