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PMID:10352183

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Citation

Haluska, P Jr, Saleem, A, Rasheed, Z, Ahmed, F, Su, EW, Liu, LF and Rubin, EH (1999) Interaction between human topoisomerase I and a novel RING finger/arginine-serine protein. Nucleic Acids Res. 27:2538-44

Abstract

The N-terminus of human topoisomerase I participates in the binding of this enzyme to helicases and other proteins. Using the N-terminal 250 amino acids of human topoisomerase I and a yeast two-hybrid/ in vitro binding screen, a novel arginine-serine-rich peptide was identified as a human topoisomerase I-binding protein. The corresponding full-length protein, named topors, contains a consensus RING zinc finger domain and nuclear localization signals in addition to the arginine-serine-rich region. The RING finger domain of topors is homologous to a similar domain in a family of viral proteins that are involved in the regulation of viral transcription. When expressed in HeLa cells as a green fluorescent protein fusion, topors localizes in the nucleus in a punctate pattern and co-immunoprecipitates with topoisomerase I. These data suggest that topors is involved in trans-cription, possibly recruiting topoisomerase I to RNA polymerase II transcriptional complexes.

Links

PubMed PMC148458

Keywords

Amino Acid Sequence; Carrier Proteins; Cell Nucleus/metabolism; DNA Topoisomerases, Type I/metabolism; DNA, Complementary/analysis; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; HeLa Cells; Humans; Molecular Sequence Data; Neoplasm Proteins; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Precipitin Tests; Saccharomyces cerevisiae; Sequence Homology, Amino Acid; Transcription Factors; U937 Cells; Ubiquitin-Protein Ligases; Zinc Fingers

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:TOP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9NS56

F

Seeded From UniProt

complete

HUMAN:TOPRS

enables

GO:0044547: DNA topoisomerase binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P11387

F

Seeded From UniProt

complete

HUMAN:TOPRS

involved_in

GO:0006351: transcription, DNA-templated

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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