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PMID:10331236
| Citation |
Horike, N and Sonobe, H (1999) Ecdysone 20-monooxygenase in eggs of the silkworm, Bombyx mori: enzymatic properties and developmental changes. Arch. Insect Biochem. Physiol. 41:9-17 |
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| Abstract |
Ecdysone 20-monooxygenase in eggs of the silkworm Bombyx mori was characterized in relation to embryonic development. First, subcellular fractions were prepared by means of differential centrifugation, and analyzed using marker enzymes and antibodies against NADPH-cytochrome P450 reductase. It was demonstrated that most ecdysone 20-monooxygenase activity was associated with microsomes, and that there was little or no intrinsic mitochondrial ecdysone 20-monooxygenase. Next, conditions for the measurement of ecdysone 20-monooxygenase activity were established for the microsomal fraction, and changes in the enzyme activity were measured in diapause eggs and non-diapause eggs during early embryogenesis. It was demonstrated that enzyme activity in diapause eggs remained at a low level, while that in the non-diapause eggs increased from the gastrula stage. The increase in egg ecdysone 20-monooxygenase activity was prevented by actinomycin D and alpha-amanitin, suggesting that gene transcription is required for eliciting an increase in ecdysone 20-monooxygenase activity. |
| Links |
PubMed Online version:<9::AID-ARCH3>3.0.CO;2-G 10.1002/(SICI)1520-6327(1999)41:1<9::AID-ARCH3>3.0.CO;2-G |
| Keywords |
Amanitins/pharmacology; Animals; Aryl Hydrocarbon Hydroxylases; Bombyx/embryology; Bombyx/enzymology; Cytochrome P-450 Enzyme System/isolation & purification; Cytochrome P-450 Enzyme System/metabolism; Dactinomycin/pharmacology; Enzyme Inhibitors/pharmacology; Female; Ovum/enzymology; Protein Synthesis Inhibitors/pharmacology; Steroid Hydroxylases/isolation & purification; Steroid Hydroxylases/metabolism; Subcellular Fractions |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005792: microsome |
ECO:0000314: |
C |
Figure 1. The activity associated with this protein was found in subcellular fractions corresponding to the microsome. In addition to this, the authors used antibodies against NADPH-cytochrome P450 reductase which was shown to inhibit the microsomal ecdysone 20-monooxygenase previously and found that the activity was inhibited (Page 11-12). |
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References
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