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PMID:10330178

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Citation

Goyal, RK, Lin, P, Kanungo, J, Payne, AS, Muslin, AJ and Longmore, GD (1999) Ajuba, a novel LIM protein, interacts with Grb2, augments mitogen-activated protein kinase activity in fibroblasts, and promotes meiotic maturation of Xenopus oocytes in a Grb2- and Ras-dependent manner. Mol. Cell. Biol. 19:4379-89

Abstract

LIM domain-containing proteins contribute to cell fate determination, the regulation of cell proliferation and differentiation, and remodeling of the cell cytoskeleton. These proteins can be found in the cell nucleus, cytoplasm, or both. Whether and how cytoplasmic LIM proteins contribute to the cellular response to extracellular stimuli is an area of active investigation. We have identified and characterized a new LIM protein, Ajuba. Although predominantly a cytosolic protein, in contrast to other like proteins, it did not localize to sites of cellular adhesion to extracellular matrix or interact with the actin cytoskeleton. Removal of the pre-LIM domain of Ajuba, including a putative nuclear export signal, led to an accumulation of the LIM domains in the cell nucleus. The pre-LIM domain contains two putative proline-rich SH3 recognition motifs. Ajuba specifically associated with Grb2 in vitro and in vivo. The interaction between these proteins was mediated by either SH3 domain of Grb2 and the N-terminal proline-rich pre-LIM domain of Ajuba. In fibroblasts expressing Ajuba mitogen-activated protein kinase activity persisted despite serum starvation and upon serum stimulation generated levels fivefold higher than that seen in control cells. Finally, when Ajuba was expressed in fully developed Xenopus oocytes, it promoted meiotic maturation in a Grb2- and Ras-dependent manner.

Links

PubMed PMC104397

Keywords

3T3 Cells; Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Calcium-Calmodulin-Dependent Protein Kinases/metabolism; Calcium-Calmodulin-Dependent Protein Kinases/physiology; Cloning, Molecular; Cytosol/metabolism; Fibroblasts/metabolism; Fluorescent Antibody Technique; GRB2 Adaptor Protein; Homeodomain Proteins/chemistry; Homeodomain Proteins/genetics; Homeodomain Proteins/physiology; Immunoblotting; Meiosis; Mice; Microinjections; Molecular Sequence Data; Oocytes/metabolism; Precipitin Tests; Proteins/physiology; Proto-Oncogene Proteins c-myc/metabolism; Recombinant Fusion Proteins; Sequence Analysis, DNA; Stem Cells; Time Factors; Tissue Distribution; Xenopus/embryology; ras Proteins/physiology; src Homology Domains/physiology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:GRB2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q91XC0

F

Seeded From UniProt

complete

MOUSE:AJUBA

involved_in

GO:0043406: positive regulation of MAP kinase activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:AJUBA

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:AJUBA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PR:Q60631

F

Seeded From UniProt

complete


See also

References

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