GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:10207104

From GONUTS
Jump to: navigation, search
Citation

Harada, H, Harada, Y, O'Brien, DP, Rice, DS, Naeve, CW and Downing, JR (1999) HERF1, a novel hematopoiesis-specific RING finger protein, is required for terminal differentiation of erythroid cells. Mol. Cell. Biol. 19:3808-15

Abstract

The AML1/core binding factor beta (CBFbeta) transcription factor is essential for definitive hematopoiesis; however, the downstream pathways through which it functions remain incompletely defined. Using a differential cloning approach to define components of this pathway, we have identified a novel gene designated HERF1 (for hematopoietic RING finger 1), whose expression during development is dependent on the presence of functional AML1/CBFbeta. HERF1 contains a tripartite RING finger-B box-alpha-helical coiled-coil domain and a C-terminal region homologous to the ret proto-oncogene-encoded finger protein. Expression of HERF1 during embryogenesis coincides with the appearance of definitive erythropoiesis and in adult mice is restricted to erythroid cells, increasing 30-fold during terminal differentiation. Importantly, inhibition of HERF1 expression blocked terminal erythroid differentiation of the murine erythroleukemia cell line MEL, whereas its overexpression induced erythroid maturation. These results suggest an important role for this protein in erythropoiesis.

Links

PubMed PMC84222

Keywords

Animals; Carrier Proteins/genetics; Cell Differentiation/genetics; Cell Line; Cloning, Molecular; Core Binding Factor Alpha 2 Subunit; DNA-Binding Proteins; Dimethyl Sulfoxide; Erythropoiesis/genetics; Gene Expression Regulation, Developmental/genetics; In Situ Hybridization; Mice; Molecular Sequence Data; Protein Structure, Secondary; Proto-Oncogene Proteins; RNA, Messenger/genetics; Tetracycline/pharmacology; Transcription Factors/genetics; Zinc Fingers/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:RUNX1

acts_upstream_of_or_within

GO:0060216: definitive hemopoiesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:1857580

P

Seeded From UniProt

complete

HUMAN:TRI15

involved_in

GO:0007500: mesodermal cell fate determination

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

MOUSE:TRI10

acts_upstream_of_or_within

GO:0030218: erythrocyte differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.