PMID:10207009

Citation	Yao, I, Hata, Y, Ide, N, Hirao, K, Deguchi, M, Nishioka, H, Mizoguchi, A and Takai, Y (1999) MAGUIN, a novel neuronal membrane-associated guanylate kinase-interacting protein. J. Biol. Chem. 274:11889-96
Abstract	Postsynaptic density (PSD)-95/Synapse-associated protein (SAP) 90 and synaptic scaffolding molecule (S-SCAM) are neuronal membrane-associated guanylate kinases. Because PSD-95/SAP90 and S-SCAM function as synaptic scaffolding proteins, identification of ligands for these proteins is important to elucidate the structure of synaptic junctions. Here, we report a novel protein interacting with the PDZ domains of PSD-95/SAP90 and S-SCAM and named it MAGUIN-1 (membrane-associated guanylate kinase-interacting protein-1). MAGUIN-1 has one sterile alpha motif, one PDZ, and one plekstrin homology domain. MAGUIN-1 is localized at the plasma membrane via the plekstrin homology domain and the C-terminal region and interacts with PSD-95/SAP90 and S-SCAM via a C-terminal PDZ domain-binding motif. MAGUIN-1 has a short isoform, MAGUIN-2, which lacks a PDZ domain-binding motif. MAGUINs are expressed in neurons and localized in the cell body and neurites and are coimmunoprecipitated with PSD-95/SAP90 and S-SCAM from rat crude synaptosome. MAGUIN-1 may play an important role with PSD-95/SAP90 and S-SCAM to assemble the components of synaptic junctions.
Links	PubMed
Keywords	Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; CHO Cells; COS Cells; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/metabolism; Cricetinae; DNA, Complementary; Guanylate Cyclase/metabolism; Guanylate Kinase; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Protein Binding; Rats; Sequence Homology, Amino Acid
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:DLG4	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	RGD:708454	F		Seeded From UniProt	complete
RAT:CNKR2	located_in	GO:0043025: neuronal cell body	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:CNKR2	located_in	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:CNKR2	located_in	GO:0045211: postsynaptic membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:CNKR2	located_in	GO:0043005: neuron projection	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0099147: extrinsic component of postsynaptic density membrane	ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion		C	part_of:(GO:0098978) part_of:(UBERON:0002421)	Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0098978: glutamatergic synapse	ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion		C	part_of:(UBERON:0002421)	Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0099147: extrinsic component of postsynaptic density membrane	ECO:0006042: cell fractionation evidence used in manual assertion		C	part_of:(GO:0098978) part_of:(UBERON:0002421)	Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0098978: glutamatergic synapse	ECO:0006042: cell fractionation evidence used in manual assertion		C	part_of:(UBERON:0002421)	Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0099147: extrinsic component of postsynaptic density membrane	ECO:0000279: qualitative western immunoblotting evidence used in manual assertion		C	part_of:(GO:0098978) part_of:(UBERON:0002421)	Seeded From UniProt	complete
RAT:CNKR2	is_active_in	GO:0098978: glutamatergic synapse	ECO:0000279: qualitative western immunoblotting evidence used in manual assertion		C	part_of:(UBERON:0002421)	Seeded From UniProt	complete

References

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