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PMID:10207009

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Citation

Yao, I, Hata, Y, Ide, N, Hirao, K, Deguchi, M, Nishioka, H, Mizoguchi, A and Takai, Y (1999) MAGUIN, a novel neuronal membrane-associated guanylate kinase-interacting protein. J. Biol. Chem. 274:11889-96

Abstract

Postsynaptic density (PSD)-95/Synapse-associated protein (SAP) 90 and synaptic scaffolding molecule (S-SCAM) are neuronal membrane-associated guanylate kinases. Because PSD-95/SAP90 and S-SCAM function as synaptic scaffolding proteins, identification of ligands for these proteins is important to elucidate the structure of synaptic junctions. Here, we report a novel protein interacting with the PDZ domains of PSD-95/SAP90 and S-SCAM and named it MAGUIN-1 (membrane-associated guanylate kinase-interacting protein-1). MAGUIN-1 has one sterile alpha motif, one PDZ, and one plekstrin homology domain. MAGUIN-1 is localized at the plasma membrane via the plekstrin homology domain and the C-terminal region and interacts with PSD-95/SAP90 and S-SCAM via a C-terminal PDZ domain-binding motif. MAGUIN-1 has a short isoform, MAGUIN-2, which lacks a PDZ domain-binding motif. MAGUINs are expressed in neurons and localized in the cell body and neurites and are coimmunoprecipitated with PSD-95/SAP90 and S-SCAM from rat crude synaptosome. MAGUIN-1 may play an important role with PSD-95/SAP90 and S-SCAM to assemble the components of synaptic junctions.

Links

PubMed

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Animals; CHO Cells; COS Cells; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Membrane/metabolism; Cricetinae; DNA, Complementary; Guanylate Cyclase/metabolism; Guanylate Kinase; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Nerve Tissue Proteins/metabolism; Neurons/metabolism; Protein Binding; Rats; Sequence Homology, Amino Acid

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:DLG4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:708454

F

Seeded From UniProt

complete

RAT:CNKR2

located_in

GO:0043025: neuronal cell body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

located_in

GO:0014069: postsynaptic density

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

located_in

GO:0045211: postsynaptic membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

located_in

GO:0043005: neuron projection

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0099147: extrinsic component of postsynaptic density membrane

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0098978: glutamatergic synapse

ECO:0006062: wide-field fluorescence microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0099147: extrinsic component of postsynaptic density membrane

ECO:0006042: cell fractionation evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0098978: glutamatergic synapse

ECO:0006042: cell fractionation evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0099147: extrinsic component of postsynaptic density membrane

ECO:0000279: qualitative western immunoblotting evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:CNKR2

is_active_in

GO:0098978: glutamatergic synapse

ECO:0000279: qualitative western immunoblotting evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete


See also

References

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