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PMID:10198254

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Citation

Thoreau, V, Bergès, T, Callebaut, I, Guillier-Gencik, Z, Gressin, L, Bernheim, A, Karst, F, Mornon, JP, Kitzis, A and Chomel, JC (1999) Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8). Biochem. Biophys. Res. Commun. 257:577-83

Abstract

We report the cloning of a cDNA encoding human syntaxin 8 (STX8), using the regulator (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) as a bait to screen a human fetal lung cDNA library by the yeast two-hybrid system. This gene was found broadly transcribed and its mRNA size is about 1.3 kb. The STX8 gene maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein. Syntaxin 8 contains in its C-terminal half a coiled-coil domain found highly conserved in the t-SNARE (SNAP receptor on target membrane) superfamily of proteins, which are involved in vesicular trafficking and docking. In syntaxin 8, a C-terminal hydrophobic domain may constitute a transmembrane anchor. It was recently shown that CFTR-mediated chloride currents can be regulated by syntaxin 1A, a t-SNARE family member, through direct protein-protein interaction. This raises the possibility that syntaxin 8 may also be involved in such regulations.

Links

PubMed Online version:10.1006/bbrc.1999.0503

Keywords

Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; Chromosome Mapping; Chromosomes, Human, Pair 17/genetics; Cloning, Molecular; Conserved Sequence; Cystic Fibrosis Transmembrane Conductance Regulator/genetics; Cystic Fibrosis Transmembrane Conductance Regulator/metabolism; Expressed Sequence Tags; Gene Expression; Humans; Lung/embryology; Lung/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Open Reading Frames/genetics; Protein Binding; Protein Structure, Secondary; Qa-SNARE Proteins; RNA, Messenger/analysis; RNA, Messenger/genetics; SNARE Proteins; Sequence Homology, Amino Acid; Syntaxin 1; Vesicular Transport Proteins; Yeasts/genetics; Yeasts/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:STX8

located_in

GO:0005887: integral component of plasma membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete

HUMAN:STX8

part_of

GO:0005887: integral component of plasma membrane

ECO:0000304: author statement supported by traceable reference used in manual assertion

C

Seeded From UniProt

complete


See also

References

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