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PMID:10103013

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Citation

Hatzi, E and Badet, J (1999) Expression of receptors for human angiogenin in vascular smooth muscle cells. Eur. J. Biochem. 260:825-32

Abstract

Human angiogenin is a plasma protein with angiogenic and ribonucleolytic activities. Angiogenin inhibited both DNA replication and proliferation of aortic smooth muscle cells. Binding of 125I-angiogenin to bovine aortic smooth muscle cells at 4 degrees C was specific, saturable, reversible and involved two families of interactions. High-affinity binding sites with an apparent dissociation constant of 0.2 nm bound 1 x 104 molecules per cell grown at a density of 3 x 104.cm-2. Low-affinity binding sites with an apparent dissociation constant of 0.1 micrometer bound 4 x 106 molecules.cell-1. High-affinity binding sites decreased as cell density increased and were not detected at confluence. 125I-angiogenin bound specifically to cells routinely grown in serum-free conditions, indicating that the angiogenin-binding components were cell-derived. Affinity labelling of sparse bovine smooth muscle cells yielded seven major specific complexes of 45, 52, 70, 87, 98, 210 and 250-260 kDa. The same pattern was obtained with human cells. Potential modulators of angiogenesis such as protamine, heparin and the placental ribonuclease inhibitor competed for angiogenin binding to the cells. Together these data suggest that cultured bovine and human aortic smooth muscle cells express specific receptors for human angiogenin.

Links

PubMed

Keywords

Animals; Aorta/metabolism; Binding, Competitive; Cattle; Cell Division/drug effects; Cells, Cultured; Humans; Iodine Radioisotopes; Muscle, Smooth, Vascular/drug effects; Muscle, Smooth, Vascular/metabolism; Proteins/metabolism; Proteins/pharmacology; Receptors, Cell Surface/biosynthesis; Recombinant Proteins; Ribonuclease, Pancreatic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BOVIN:ANG1

involved_in

GO:0048662: negative regulation of smooth muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:Q5WRG2

involved_in

GO:0007202: activation of phospholipase C activity

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ANGI

enables

GO:0004519: endonuclease activity

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:ANGI

involved_in

GO:0048662: negative regulation of smooth muscle cell proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:ANGI

enables

GO:0008201: heparin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:ANGI

involved_in

GO:0007154: cell communication

ECO:0000303: author statement without traceable support used in manual assertion

P

Seeded From UniProt

complete

See also

References

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