GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:10101119

From GONUTS
Jump to: navigation, search
Citation

Moore, AW, McInnes, L, Kreidberg, J, Hastie, ND and Schedl, A (1999) YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis. Development 126:1845-57

Abstract

The Wilms' Tumour gene WT1 has important functions during development. Knock-out mice were shown to have defects in the urogenital system and to die at embryonic day E13.5, probably due to heart failure. Using a lacZ reporter gene inserted into a YAC construct, we demonstrate that WT1 is expressed in the early proepicardium, the epicardium and the subepicardial mesenchymal cells (SEMC). Lack of WT1 leads to severe defects in the epicardial layer and a concomitant absence of SEMCs, which explains the pericardial bleeding and subsequent embryonic death observed in Wt1 null embryos. We further show that a human-derived WT1 YAC construct is able to completely rescue heart defects, but only partially rescues defects in the urogenital system. Analysis of the observed hypoplastic kidneys demonstrate a continuous requirement for WT1 during nephrogenesis, in particular, in the formation of mature glomeruli. Finally, we show that the development of adrenal glands is also severely affected in partially rescued embryos. These data demonstrate a variety of new functions for WT1 and suggest a general requirement for this protein in the formation of organs derived from the intermediate mesoderm.

Links

PubMed

Keywords

Adrenal Glands/embryology; Animals; Chromosomes, Artificial, Yeast; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Embryonic and Fetal Development; Fetal Heart/physiology; Gene Expression Regulation, Developmental; Genes, Reporter; Genes, Wilms Tumor; Genetic Complementation Test; Mesoderm/physiology; Mice; Mice, Knockout; Nephrons/embryology; Pericardium/embryology; Transcription Factors/genetics; Transcription Factors/physiology; WT1 Proteins; beta-Galactosidase/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:WT1

involved_in

GO:0061032: visceral serous pericardium development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0072284: metanephric S-shaped body morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0032835: glomerulus development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0030325: adrenal gland development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0007507: heart development

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

HUMAN:WT1

involved_in

GO:0001658: branching involved in ureteric bud morphogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P22561

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0001658: branching involved in ureteric bud morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0007507: heart development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0030325: adrenal gland development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0032835: glomerulus development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0043066: negative regulation of apoptotic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0061032: visceral serous pericardium development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:WT1

involved_in

GO:0072284: metanephric S-shaped body morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.