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PMID:10094048

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Citation

Li, SJ and Hochstrasser, M (1999) A new protease required for cell-cycle progression in yeast. Nature 398:246-51

Abstract

In eukaryotes, protein function can be modulated by ligation to ubiquitin or to ubiquitin-like proteins (Ubl proteins). The vertebrate Ubl protein SUMO-1 is only 18% identical to ubiquitin but is 48% identical to the yeast protein Smt3. Both SUMO-1 and Smt3 are ligated to cellular proteins, and protein conjugation to SUMO-1/Smt3 is involved in many physiological processes. It remained unknown, however, whether deconjugation of SUMO-1/Smt3 from proteins is also essential. Here we describe a yeast Ubl-specific protease, Ulp1, which cleaves proteins from Smt3 and SUMO-1 but not from ubiquitin. Ulp1 is unrelated to any known deubiquitinating enzyme but shows distant similarity to certain viral proteases, indicating the existence of a widely conserved protease fold. Proteins related to Ulp1 are present in many organisms, including several human pathogens. The pattern of Smt3-coupled proteins in yeast changes markedly throughout the cell cycle, and specific conjugates accumulate in ulp1 mutants. Ulp1 has several functions, including an essential role in the G2/M phase of the cell cycle.

Links

PubMed Online version:10.1038/18457

Keywords

Carrier Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/isolation & purification; Cell Cycle Proteins/metabolism; Cloning, Molecular; Cysteine Endopeptidases/genetics; Cysteine Endopeptidases/isolation & purification; Cysteine Endopeptidases/metabolism; Escherichia coli; Fungal Proteins/isolation & purification; Fungal Proteins/metabolism; G2 Phase/physiology; Humans; Mitosis/physiology; Molecular Sequence Data; Mutagenesis; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Repressor Proteins/metabolism; SUMO-1 Protein; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Small Ubiquitin-Related Modifier Proteins; Substrate Specificity; Ubiquitins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:ULP1

involved_in

GO:0016926: protein desumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ULP1

involved_in

GO:0016926: protein desumoylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ULP1

involved_in

GO:0000086: G2/M transition of mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

YEAST:ULP1

enables

GO:0008234: cysteine-type peptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:ULP1

enables

GO:0070140: SUMO-specific isopeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

YEAST:ULP1

enables

GO:0070140: SUMO-specific isopeptidase activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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