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PMID:10064581

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Citation

Iwasaki, H, Taniguchi, Y, Ishiura, M and Kondo, T (1999) Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria. EMBO J. 18:1137-45

Abstract

The kai gene cluster, which is composed of three genes, kaiA, kaiB and kaiC, is essential for the generation of circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Here we demonstrate the direct association of KaiA, KaiB and KaiC in yeast cells using the two-hybrid system, in vitro and in cyanobacterial cells. KaiC enhanced KaiA-KaiB interaction in vitro and in yeast cells, suggesting that the three Kai proteins were able to form a heteromultimeric complex. We also found that a long period mutation kaiA1 dramatically enhanced KaiA-KaiB interaction in vitro. Thus, direct protein-protein association among the Kai proteins may be a critical process in the generation of circadian rhythms in cyanobacteria.

Links

PubMed PMC1171205 Online version:10.1093/emboj/18.5.1137

Keywords

Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Circadian Rhythm; Circadian Rhythm Signaling Peptides and Proteins; Cyanobacteria/physiology; Gene Expression Regulation, Bacterial/genetics; Genes, Reporter; Molecular Sequence Data; Mutation; Promoter Regions, Genetic/genetics; Protein Binding; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/genetics; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SYNE7:KAIC

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF5

F

Seeded From UniProt

complete

SYNE7:KAIC

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF6

F

Seeded From UniProt

complete

SYNE7:KAIB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF4

F

Seeded From UniProt

complete

SYNE7:KAIB

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF6

F

Seeded From UniProt

complete

SYNE7:KAIA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF4

F

Seeded From UniProt

complete

SYNE7:KAIA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF5

F

Seeded From UniProt

complete

SYNE7:KAIC

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF4

F

Seeded From UniProt

complete

SYNE7:KAIC

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

SYNE7:KAIA

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

SYNE7:KAIA

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF6

F

Seeded From UniProt

complete

SYNE7:KAIB

enables

GO:0042802: identical protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q79PF5

F

Seeded From UniProt

complete

SYNE7:KAIB

involved_in

GO:0009649: entrainment of circadian clock

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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