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PMID:10051443

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Citation

St-Denis, JF, Cabaniols, JP, Cushman, SW and Roche, PA (1999) SNAP-23 participates in SNARE complex assembly in rat adipose cells. Biochem. J. 338 ( Pt 3):709-15

Abstract

SNARE proteins are required for vesicle docking and fusion in eukaryotic cells in processes as diverse as homotypic membrane fusion and synaptic vesicle exocytosis [SNARE stands for SNAP receptor, where SNAP is soluble NSF attachment protein]. The SNARE proteins syntaxin 4 and vesicle-associated membrane protein (VAMP) 2/3 also participate in the insulin-stimulated translocation of GLUT4 from intracellular vesicles to the plasma membrane in adipose cells. We now report the molecular cloning and characterization of rat SNAP-23, a ubiquitously expressed homologue of the essential neuronal SNARE protein SNAP-25 (synaptosomal-associated protein of 25 kDa). Rat SNAP-23 is 86% and 98% identical respectively to human and mouse SNAP-23. Southern blot analysis reveals that the rat, mouse and human SNAP-23 genes encode species-specific isoforms of the same protein. Co-immunoprecipitation of syntaxin 4 and SNAP-23 shows association of these two proteins in rat adipose cell plasma membranes, and insulin stimulation does not alter the SNAP-23/syntaxin 4 complex. In addition, we demonstrate for the first time the participation of SNAP-23, along with syntaxin 4 and VAMP2/3, in the formation of 20S SNARE complexes prepared using rat adipose cell membranes and recombinant alpha-SNAP and NSF proteins. The stoichiometry of the SNARE complexes formed is essentially identical using membranes from either unstimulated or insulin-stimulated adipose cells. These data demonstrate that rat SNAP-23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT4 from intracellular vesicles to the plasma membrane of rat adipose cells.

Links

PubMed PMC1220107

Keywords

Adipocytes/metabolism; Amino Acid Sequence; Animals; Biological Transport; Blotting, Southern; Carrier Proteins/chemistry; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cloning, Molecular; DNA, Complementary; Glucose Transporter Type 4; Humans; Male; Membrane Proteins/metabolism; Mice; Molecular Sequence Data; Monosaccharide Transport Proteins/metabolism; Muscle Proteins; Qa-SNARE Proteins; Qb-SNARE Proteins; Qc-SNARE Proteins; Rats; Rats, Sprague-Dawley; SNARE Proteins; Sequence Homology, Amino Acid; Subcellular Fractions/metabolism; Vesicular Transport Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

RAT:SNP23

enables

GO:0019905: syntaxin binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:621019

F

Seeded From UniProt

complete

RAT:SNP23

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:SNP23

part_of

GO:0031201: SNARE complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:SNP23

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:GLUT4

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:GLUT4

located_in

GO:0043231: intracellular membrane-bounded organelle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP3

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP3

located_in

GO:0043231: intracellular membrane-bounded organelle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP2

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP2

located_in

GO:0043231: intracellular membrane-bounded organelle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP2

part_of

GO:0031201: SNARE complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP2

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:STX4

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:STX4

part_of

GO:0031201: SNARE complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:STX4

enables

GO:0000149: SNARE binding

ECO:0000353: physical interaction evidence used in manual assertion

RGD:620221

F

Seeded From UniProt

complete

RAT:STX4

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

RAT:VAMP3

part_of

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP3

part_of

GO:0031201: SNARE complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP3

part_of

GO:0043231: intracellular membrane-bounded organelle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

RAT:VAMP3

involved_in

GO:0065003: protein-containing complex assembly

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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