PMID:10049767

Citation	Tanahashi, H and Tabira, T (1999) X11L2, a new member of the X11 protein family, interacts with Alzheimer's beta-amyloid precursor protein. Biochem. Biophys. Res. Commun. 255:663-7
Abstract	We screened proteins for interaction with Alzheimer's beta-amyloid precursor protein (APP) and cloned a new member of the X11 protein family, X11L2. The PID/PTB element of X11L2 protein interacted with the intracellular domain of APP by GST binding assay, and in vivo interaction was confirmed by coimmunoprecipitation from cell extracts overexpressing APP and HA-tagged X11L2. This gene encoded 575 amino acids and the deduced amino acid sequence was highly homologous to rat Mint3. Three protein-protein interaction domains, a PID/PTB and two PDZ elements, were conserved among the X11 protein family, and the N-terminal region of X11L2 protein had several putative SH3 binding motifs, PXXP. Unlike other members of the X11 protein family, X11L2 mRNA was expressed in various tissues.
Links	PubMed Online version:10.1006/bbrc.1999.0265
Keywords	Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Amyloid beta-Protein Precursor/metabolism; Carrier Proteins; Cloning, Molecular; Conserved Sequence/genetics; Humans; Membrane Proteins; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Protein Binding/genetics; Proteins/chemistry; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Sequence Alignment; Sequence Analysis, DNA; src Homology Domains/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:APBA3	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:P05067	F		Seeded From UniProt	complete
HUMAN:A4	enables	GO:0005515: protein binding	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O96018	F		Seeded From UniProt	complete

References

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