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PMID:10049767

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Citation

Tanahashi, H and Tabira, T (1999) X11L2, a new member of the X11 protein family, interacts with Alzheimer's beta-amyloid precursor protein. Biochem. Biophys. Res. Commun. 255:663-7

Abstract

We screened proteins for interaction with Alzheimer's beta-amyloid precursor protein (APP) and cloned a new member of the X11 protein family, X11L2. The PID/PTB element of X11L2 protein interacted with the intracellular domain of APP by GST binding assay, and in vivo interaction was confirmed by coimmunoprecipitation from cell extracts overexpressing APP and HA-tagged X11L2. This gene encoded 575 amino acids and the deduced amino acid sequence was highly homologous to rat Mint3. Three protein-protein interaction domains, a PID/PTB and two PDZ elements, were conserved among the X11 protein family, and the N-terminal region of X11L2 protein had several putative SH3 binding motifs, PXXP. Unlike other members of the X11 protein family, X11L2 mRNA was expressed in various tissues.

Links

PubMed Online version:10.1006/bbrc.1999.0265

Keywords

Adaptor Proteins, Signal Transducing; Amino Acid Sequence; Amyloid beta-Protein Precursor/metabolism; Carrier Proteins; Cloning, Molecular; Conserved Sequence/genetics; Humans; Membrane Proteins; Molecular Sequence Data; Nerve Tissue Proteins/chemistry; Nerve Tissue Proteins/genetics; Protein Binding/genetics; Proteins/chemistry; RNA, Messenger/metabolism; Recombinant Fusion Proteins/genetics; Sequence Alignment; Sequence Analysis, DNA; src Homology Domains/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:APBA3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P05067

F

Seeded From UniProt

complete

HUMAN:A4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O96018

F

Seeded From UniProt

complete


See also

References

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